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Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development

ABSTRACT: Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development

PROVIDER: PRJNA1064870 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27548960_1.fastq.gz	Fastqsanger.gz
	SRR27548960_2.fastq.gz	Fastqsanger.gz
	SRR27548961_1.fastq.gz	Fastqsanger.gz
	SRR27548961_2.fastq.gz	Fastqsanger.gz
	SRR27548962_1.fastq.gz	Fastqsanger.gz

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Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development

Project description:Parasympathetic neurons (parasymNs) belong to the autonomic nervous system (ANS) and are critical for unconscious body responses, including rest-and-digest and calming the body. ParasymN dysfunction has been found involved in neural diseases such as autonomic neuropathy and neurological autoimmune disease; and parasymN innervation is important for organ development. However, human parasymN function and dysfunction is vastly understudied, due to the lack of a model system. Human pluripotent stem cell (hPSC)-derived neurons can fill this void and serve for disease modeling, drug screening, and transplantation therapy. Here, we developed a differentiation paradigm detailing for the first time, the derivation of functional human parasymNs from Schwann cell progenitors (SCP). We employ these neurons (i) to assess human ANS development paradigms, (ii) to model neuropathy in the genetic disorder Familial Dysautonomia (FD), (iii) to show parasymN dysfunction during SARS-CoV-2 infection, (iv) to model the autoimmune disease Sjörgen’s syndrome and, (v) to show that parasymN innervation of white adipocytes during development and help mature the tissue. Our model system will become instrumental for future disease mechanistic and drug discovery studies as well as for human developmental studies.

2024-01-15 | GSE253235 | GEO

Parasympathetic neurons derived from human pluripotent stem cells model human diseases and development.

Project description: Not available

| S-EPMC11069445 | biostudies-literature

Differentiation of human induced pluripotent stem cells into induced motor neurons

Project description:Characterisation of induced motor neurons (iMN) generated from human induced pluripotent stem cells (hiPSC, hPSCreg name BIHi005-A) using doxycycline inducible expression of Ngn2, Isl1 and Lhx3. While this method of iMN generation has been described before (REF: doi.org/10.1002/cpcb.51), no extensive characterisation of the transcriptome and proteome throughout the differentiation process or of the mature neurons has been undertaken so far.

2025-11-24 | PXD031066 | Pride

Generation of Locus Coeruleus Norepinephrine Neurons from Human Pluripotent Stem Cells [snRNA-seq]

Project description:Central norepinephrine (NE) neurons, mainly located in the Locus coeruleus (LC), play roles in a wide range of behavioral and physiological processes. How the human LC-NE neurons develop and what roles they play in the pathophysiology of human diseases is poorly understood, partly due to the unavailability of functional human LC-NE neurons. Here we established a technology for efficient generation of LC-NE neurons from human pluripotent stem cells by identifying a novel role of ACTIVIN A in regulating the LC-NE transcription factors in the dorsal rhombomere 1 (r1) progenitors. The in vitro generated human LC-NE neurons not only display extensive axonal arborization and release/uptake NE, but also exhibit the pacemaker activity, calcium oscillation, and in particular chemoreceptor activity in response to CO2. Multiple timepoint single nucleus RNA-Seq (snRNA-Seq) analysis captured the dynamic NE differentiation process, confirmed the NE cell population and revealed the differentiation trajectory from hindbrain progenitors to NE neurons via ASCL1 expressing precursor stage. The LC-NE neurons engineered with a NE sensor reliably reported the extracellular NE level. The availability of functional human LC-NE neurons enables investigation of their roles in the pathogenesis of and development of therapeutics for neural psychiatric and degenerative diseases.

2023-08-24 | GSE221988 | GEO

Total RNA sequencing for human induced pluripotent derived cortical neurons

Project description:Total RNA sequencing for human induced pluripotent derived cortical neurons

| PRJNA690236 | ENA

Total RNA sequencing for human induced pluripotent derived cortical neurons

Project description:Total RNA sequencing for human induced pluripotent derived neurons organoids from healthy controls and schizophrenia (SCZ) patients

2021-01-08 | GSE164376 | GEO

Somatic mutations in Single Human Neurons from Patients with Congenital Neurodegenerative Diseases

Project description:Somatic mutations in Single Human Neurons from Patients with Congenital Neurodegenerative Diseases

| PRJNA864078 | ENA

Somatic mutations in Single Human Neurons from Patients with Congenital Neurodegenerative Diseases

Project description:Somatic mutations in Single Human Neurons from Patients with Congenital Neurodegenerative Diseases

| PRJNA864079 | ENA

RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells

Project description:RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells

| PRJEB32451 | ENA

in-vitro differentiated midbrain dopaminergic neurons from isogenic human induced pluripotent stem cells

Project description:We have generated isogenic induced pluripotent stem cell lines by reprogramming human fibroblasts from patients carrying the LRRK2 G2019S mutation with subsequent zinc finger nuclease - mediated targeted correction of the diseased allele. These iPS cell lines were differentiated for 30 days using a direct differentiation protocol towards midbrain dopaminergic neurons (mDANs). Isogenic human iPS cells carrying the LRRK2 WT and G2019S locus were differentiated to dopaminergic neurons to detect gene expression changes associated with mutated LRRK2.

2013-03-07 | E-GEOD-43364 | biostudies-arrayexpress

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