Project description:This submission is a dataset of two modalities, single-nucleus transcriptomics and single-nuclei spatial transcriptomics in the spinal cords of mice. The single-nuclei transcriptomics data is harvested and profiled using 10x Genomics Chromium Single Cell Kit Version. The single-nuclei spatial transcriptomics data is harvested and profiled using Visium Spatial Gene Expression.
Project description:We profiled human lung and breast tumor fragments using imaging-based single-cell spatial transcriptomics (10x Xenium) and compared the results to sequencing-based spatial transcriptomics (10x Visium) and single-nucleus RNA-seq
Project description:This submission is a dataset of single-nucleus transcriptomics in the spinal cords of mice. The single-nuclei transcriptomics data is harvested and profiled using Visium Spatial Gene Expression.
Project description:These data were used in the spatial transcriptomics analysis of the article titled \\"Single-Cell and Spatial Transcriptomics Analysis of Human Adrenal Aging\\".
Project description:To uncover molecular determinants of motor neuron degeneration and selective vulnerability in amyotrophic lateral sclerosis (ALS), we performed longitudinal single-nucleus RNA sequencing, paired single-nucleus RNA and ATAC sequencing, and MERFISH spatial transcriptomics from control and SOD1-G93A mouse spinal cords. For the final snRNA-seq analysis, control experiments from Blum et al., Nat Neurosci, 2021 (GEO accession: GSE161621) were also used.
Project description:We investigated neural populations mediating the effects of cagrilintide on energy balance in the dorsal vagal complex (DVC). Single-nucleus RNA sequencing and spatial transcriptomics were performed across mouse, rat, and macaque brainstem tissue. Treatment groups include diet-induced obese animals treated acutely or subchronically with cagrilintide or vehicle. The overarching aim was to identify and characterize the neuronal population showing the strongest and most sustained transcriptional response to treatment. Included in this ArrayExpress ID is the single-cell data for mouse, rat and macaque
Project description:By combining single nucleus transcriptomics with spatial gene expression analysis and high-resolution electron microscopy, we reconstructed the cell type atlas of post-metamorphic Paracentrotus lividus juveniles.
Project description:Identification of cell types in the interphase between muscle and tendon by Visium Spatial Transcriptomics of four human semitendinous muscle-tendon biopsies. Cell types identified by single nuclei RNA seq on similar tissue were localized in situ with the use of Spatial Transcriptomics.
Project description:We profiled 87 primary-recurrentpatient-matched paired GBM specimens with single-nucleus RNA and bulk-DNA sequencing and single-cell open-chromatin and spatial transcriptomics/proteomics assays. We found that recurrent GBMs are characterized by a shift to a mesenchymal phenotype in response to therapy
Project description:We investigated neural populations mediating the effects of cagrilintide on energy balance in the dorsal vagal complex (DVC). Single-nucleus RNA sequencing and spatial transcriptomics were performed across mouse, rat, and macaque brainstem tissue. Treatment groups include diet-induced obese animals treated acutely or subchronically with cagrilintide or vehicle. The overarching aim was to identify and characterize the neuronal population showing the strongest and most sustained transcriptional response to treatment. Included in this ArrayExpress ID is the bulk-seq data for mouse and rat
