Candida albicans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA106527 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of Candida albicans after 3 h phagocytosis by vehicle DMSO-treated macrophages (intact, expanding phagosomes) or calcium chelator BAPTA-AM-treated macrophages (inhibits lysosomal repair of expanding phagosomes, leading to phagosome rupture) to determine the effect of preventing phagosome expansion on C. albicans gene expression after phagocytosis by macrophages. Cultivation of Candida only for 3 h in DMEM-FBS cell culture medium or YPD complex medium as non-phagocytosis control conditions.
2020-10-05 | GSE147207 | GEO
Project description:To investigate the effect of METRNL on macrophages phagocytosis and killing of Candida albicans, we designed RNA-Seq analysis of macrophages pretreated with vehicle or rmMETRNL challenged with Candida albicans for 6 hours in vitro.
2026-01-28 | GSE290122 | GEO
Project description:To investigate the effect of progranulin on macrophages phagocytosis and killing of Candida albicans, we designed RNA-Seq analysis of wild-type and PGRN-/- macrophages challenged with Candida albicans in vitro at one time point.
2022-09-13 | GSE201165 | GEO
Project description:The effects of the SCFA crotonate on fungal and host transcriptomes were addressed, following infection of mouse bone marrow-derived macrophages(BMDMs) with Candida albicans.
2023-10-02 | GSE234554 | GEO
Project description:Purpose: The purpose of this study was to simulataneously examine the host and fungal pathogen transcriptional profiles of four distinct infection fates during macrophage and Candida albicans interactions Methods: Membrane stained (Deep Red),primary, bone marrow derived, murine macrophages and Candida albicans expressing GFP and mCherry were exposed to each other over a four hour time course. Samples were collected at 0, 1, 2 and 4 hours and sorted for four infection subpopulations: 1. Macrophages which phagocytosed live C. albicans (GFP+ /mCherry+ /Deep Red +), 2. Macrophages which phagocytosed dead C. albicans (GFP- /mCherry+ /Deep Red +), 3. Uninfected macrophages(GFP- /mCherry- /Deep Red +) and 4. Unengulfed C. albicans (GFP+ /mCherry + /Deep Red -). Unexposed controls were also collected for some time points (i.e. macrophages never exposed to C. albicans and C. albicans never exposed to macrophages). Single macrophages infected with live or dead C. albicans were also sorted. Smart-seq2 was used to create libraries for both infection subpopulation and single, infected cell samples that were sequenced on Illumina’s Miseqand Nextseq. Basic quality assessment of Illumina reads and sample demultiplexing was done with Picard version 1.107 and Trimmomatic. Samples profiling exclusively the mouse transcriptional response were aligned to the mouse transcriptome generated from the v. Dec. 2011 GRCm38/mm10 and a collection of mouse rRNA sequences from the UCSC genome website. Samples profiling exclusively the yeast transcriptional response were aligned to the C. albicans transcriptome strain SC5314 version A21-s02-m09-r10 downloaded from Candida Genome Database. Samples containing both macrophages and C. albicans were aligned to a “composite transcriptome” made by combining the mouse transcriptome and C. albicans transcriptomes described above and alignment was done via BWA (version 0.7.10-r789.) Multi-reads (reads that aligned to both host and pathogen transcripts) were discarded. Then, each host or pathogen sample file were aligned to its corresponding reference using Bowtie2 and RSEM (v.1.2.21). Transcript abundance was estimated using transcripts per million (TPM). For subpopulation samples, TPM was calculated using edgeR, all as implemented in the Trinity package version 2.1.. Genes were considered differentially expressed only if they had a 4-fold change difference (> 4 FC) in TPM values and a false discovery rate below or equal to 0.001 (FDR < 0.001), unless specified otherwise. For single macrophages infected with C. albicans, samples were aligned to the combined transcriptome as described above and RSEM was used to calculate TPM. Results: We were able to simultaneously measure the host and fungal pathogen transcriptional profiles of four distinct infection fates during macrophage and Candida albicans interactions Conclusions: Our study represents an analysis of both distinct infection populations of macrophages and fungus.
2018-03-12 | GSE111731 | GEO
Project description:Transcriptional response of Candida albicans to endothelial cells
| PRJNA96201 | ENA
Project description:Transcriptional response of Candida albicans to oral epithelial cells
| PRJNA96243 | ENA
Project description:To investigate the effect of Progranulin on neutrophils phagocytosis and killing of Candida albicans, we designed RNA-Seq analysis of wild-type and PGRN-/- neutrophils challenged with Candida albicans in vitro at one time point.
2022-09-13 | GSE201166 | GEO
Project description:Candida albicans is a commensal fungi which populates most healthy individuals’ microflora, but can turn opportunistic in immunocompromised individuals. This opportunistic pathogen can cause severe disease and is linked with high rates of mortality. With limited therapeutic options and increasing resistance to antifungals, novel treatment strategies for C. albicans infections is paramount. Macrophages are a key immune cell which mount an immediate response to C. albicans infection. Macrophages physically sequester C. albicans through phagocytosis, trapping the pathogen in a harsh environment. Additionally, macrophages mount a signalling response, alerting other immune cells to the site of infection for quick removal. Here we utilise a discovery screen approach using DIA based total proteomics to describe murine bone marrow derived macrophage (BMDM) response to C. albicans infection as well as in response to co-infection with gram-negative bacterial plasma membrane component lipopolysaccharide (LPS) or live gram-negative bacteria Pseudomonas aeruginosa. We found C. albicans induced a surprisingly muted response in BMDMs compared to LPS or P. aeruginosa. Despite small measurable activation of BMDMs on single infection, upon co-infection with LPS or P. aeruginosa, C. albicans significantly suppressed BMDM proteome landscape. Additionally, C. albicans selectively suppressed BMDM secreted IL-6 and IL-12p40 cytokine response to P. aeruginosa. Thus, we describe C. albicans significant suppressive capabilities in host innate immune responses.
2026-03-16 | PXD062689 | Pride