Project description:The aim of this study was to characterize the molecular interactions zone between the plerocercoid of Ligula intestinalis and its host, a common bream Abramis brama. Using HPLC-MS/MS we performed a comparative proteomic analysis of washes from the parasite surface and from the body cavity of infected and uninfected fish. We identified both parasitic and host proteins on the helminth surface, reflecting an active molecular crosstalk. Functional analysis revealed cestode-specific metabolic and signaling pathways (eg. such as proline biosynthesis and TOR signaling), including those for amino acid biosynthesis, lipid transport, and nutrient sensing. Within the body cavity of infected fish, we detected parasite-derived proteins such as immunomodulators, proteases, and metabolic enzymes. Quantitative changes in host wash proteomes indicated activation of humoral immunity alongside remodeling of proteolytic regulation. Collectively, these data reveal the molecular foundation of L. intestinalis adaptation and the dynamic host-parasite relationships in this system.
Project description:Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and ageing of a basal chordate. Keywords: gene expression, development, ageing, life cycle
Project description:We report the comprehensive sequencing of small RNA libraries created from different developmental stages (larva and gastrula) of the basal chordate, Ciona intestinalis. These libraries were used for the identification of microRNAs in this organism. Sequencing of small RNA libraries from 2 stages of Ciona intestinalis.
Project description:We report the comprehensive sequencing of small RNA libraries created from different developmental stages (larva and gastrula) of the basal chordate, Ciona intestinalis. These libraries were used for the identification of microRNAs in this organism.
