Project description:mRNA samples from bone marrow CD34+ cells (HSC) from healthy donors after co-culture assays (Control condition, with hMSCs and CD34+ cells from healthy donors; and AML condition, with hMSCs-AML and CD34+ cells from donors) were amplified, labeled and hybridized to the ClariomTM S Array human (Thermo Scientific, USA). Normalization and analysis of microarray data was performed using the Transcriptome Analysis Console (TAC) software (Affymetrix, USA).
Project description:Transcriptome of young HSCs (Lin-CD34+CD38-), young Progenitors (Lin-CD34+CD38+) and old HSCs (Lin-CD34+CD38-) isolated from bone marrow samples of healthy donors.
Project description:To evaluate the transcriptome and splicing repertoire of bulk CD34+ sorted bone marrow progenitors we performed RNA-Seq. These data highlight an AML prognostic splicing signature that is present in healthy donors at equivalent frequencies to that found in AML bone marrow.
Project description:RNA-seq of bone marrow samples of chronic myelomonocytic leukemia (CMML) patients and healthy donors to identify the molecular DNA repair pathways involved in CMML pathogenic
Project description:AC133+ cell fractions were isolated from bone marrow of 33 acute myeloid leukemia patients and 10 healthy donors and their transcriptional profiles assessed with Affymetrix GeneChip Human Genome U133 Plus 2.0
Project description:Microarray analysis of bone marrow multipotent mesenchymal stromal cells isolated from type 1 diabetes patients and healthy donors.