Project description:Complex gut microbiota increases chicken resistance to enteric diseases. However, the principles of this phenomenon are not understood in detail. One of the possibilities how to decipher the role of gut microbiota in chicken resistance to enteric pathogens is to gradually characterise gene expression of individual gut microbiota members when colonising chicken caecum. To reach this aim, newly hatched chicks were inoculated with bacterial species with known whole genomic sequence. Total protein purified from the chicken caecum was analysed by mass spectrometry and obtained spectra were searched against strain-specific protein databases generated from known genomic sequences.
