Project description:Transcriptome sequencing of Foxtail millet Setaria italica (Zhang-gu) for different tissues. Four RNA pools were created corresponding to four different tissues: root, leaf, stem, spica (tassel) at developmental stage, then each pool was sequenced.
Project description:the SiNRX1 of foxtail millet was knocked out by means of the CRISPR/Cas9 technology, and the drought resistance of SiNRX1 was identified at both the germination stage and the seedling stage. Moreover, through transcriptome sequencing and Data-independent acquisition (DIA) quantitative proteomics determination of sinrx1 mutants and wild types (WT) at the seedling stage under drought and control conditions, the molecular mechanism of SiNRX1 regulating drought resistance was preliminarily analyzed.
Project description:N6-methyladenosine (m6A) is a pivotal epitranscriptomic modification that regulates mRNA metabolism and plays critical roles in plant growth and environmental responses. However, the evolution and roles of m6A modifications in plant domestication remain largely unexplored. This study combined RNA-seq and MeRIP-seq data to compare m6A modifications profiles between foxtail millet (Setaria italica, Yugu1) and its wild ancestor S. viridis (A10). Peak calling analysis identified 6,928 m6A peaks associated with 6,407 protein-coding genes in Yugu1 and 6,274 m6A peaks in 5,858 genes in A10, respectively. Comparative analysis revealed numerous differentially expressed genes (DEGs) and differentially methylated peaks (DMPs), whose functions were elucidated by GO and KEGG enrichment analyses. Integration of DMPs paired with DEGs, we found 209 hyper-methylated peaks associated with upregulated transcription levels, and 90 hypo-methylated peaks associated with downregulated transcription levels. This dataset provides valuable resource for further investigating the roles of m⁶A modification in crop domestication.
