Project description:NILs containing five parental lines, three wild barley genotypes ssp. spontaneum: HID 4 (A), Iraq; HID 64 (B), Turkey; and HID 369 (C), Israel, one ssp. agriocrithon: HID 382(D)) and cv. Morex (ssp. vulgare, USA). Purpose: Variant calling to identifie markers associated with a awn length QTL on the distal part of chromosome 7HL
Project description:The molecular regulation of root initiation in monocots is still poorly understood. We report the development of the auxin-based crown-root inducible system (CRIS) in barley (Hordeum vulgare L.). Using this system coupled to RNAseq, we investigated the molecular events occurring during the early steps of crown-root (CR) initiation. The sequencing on the Illumina NovaSeq6000 platform yielded over 123 Gb data, representing more than 408 million reads. Using the last released barley annotation (Morex v2 Gene Models, 2019), we found that 427 genes were downregulated by auxin whereas 764 genes were upregulated. This study provides the first analysis of early event of crown-root initiation in cereals. It will serve as comparison with known genes in rice, maize and other cereals, and could be used in metagenomics studies.
