Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Sea buckthorn dieback in Northern Germany: Fungal isolations and mycobiome analyses

PROVIDER: PRJNA1069732 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27743100_1.fastq.gz	Fastqsanger.gz
	SRR27743100_2.fastq.gz	Fastqsanger.gz
	SRR27743200_1.fastq.gz	Fastqsanger.gz
	SRR27743200_2.fastq.gz	Fastqsanger.gz
	SRR27743300_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 628

Similar Datasets

Project description:Sea buckthorn fungal microbiota

| PRJNA590349 | ENA

TMT-based Quantitative Proteomics of Developing Sea Buckthorn (Hippophae rhamnoides) Berries Reveals Candidate Proteins Related to Lipid Metabolism

Project description:Sea buckthorn (Hippophae rhamnoides L.) is an economically important shrub or small tree indigenous to Asia and Europe. The most well-recognized medicinal and nutraceutical products derive from the berry oil, which is rich in essential fatty acids, monounsaturated palmitoleic acid (16:1 ω-7), linoleic (18:2 ω-6) and alpha-linolenic (18:3 ω-3) acids. In this study, tandem mass tags (TMT) MS/MS were used to investigate protein profiles of lipid metabolism in sea buckthorn berries harvested at 30, 50 and 70 days after flowering. A total of 8626 proteins were detected and 6170 of these were quantified. Further analyses of the proteins and their pathways revealed initiation of fatty acid accumulation throughout the whole berry development. Activity of most of key enzymes, which are related to biosynthesis pathways of triacylglycerol and fatty acids, had peaked at 50 days; but triacylglycerol synthesis through the PDAT (phospholipid: diacylglycerol acyltransferase) catalytic pathway of occurred mostly at the early stage of berry development. Protein expression patterns related to lipid accumulation were also verified at the transcript level using quantitative real-time polymerase chain reaction. The proteome profiles determined here will improve our understanding of the process of lipid accumulation and the dynamic changes in metabolic pathways during sea buckthorn berry development.

2019-04-18 | PXD009365 | Pride

Multi-omic data reveals the role of the antioxidant network in the quality of growing sea buckthorn fruit

Project description:Fruits of sea buckthorn have abundant antioxidants and attractive colors. They provide excellent materials to study the relationships among fruit color, antioxidant and fruit quality

2022-03-03 | PXD004251 | Pride

Hippophae rhamnoides

Project description:sea buckthorn fruit lncRNAs

| PRJNA325699 | ENA

Proteome profiling reveals key insights about the mechanisms under low-temperature stress in sea buckthorn

Project description:Sea buckthorn (Hippophae rhamnoides L.) is well adapted to extreme temperatures ranging from −43 to 40°C and is ideal material to study mechanisms related to low-temperature stress tolerance

2018-10-27 | PXD004609 | Pride

Project description:Leaf transcriptome of sea buckthorn

| PRJNA642290 | ENA

Hippophae rhamnoides

Project description:WGS of sea buckthorn genotypes

| PRJNA1177110 | ENA

Exploring the female genital tract mycobiome in young South African women using metaproteomics

Project description:Female genital tract (FGT) diseases such as bacterial vaginosis (BV) and sexually transmitted infections are prevalent in South Africa, with young women being at an increased risk. Since imbalances in the FGT microbiome are associated with FGT diseases, it is vital to investigate the factors that influence FGT health. The mycobiome plays an important role in regulating mucosal health, especially when the bacterial component is disturbed. However, we have a limited understanding of the FGT mycobiome since many studies have focused on bacterial communities and have neglected low abundance taxonomic groups, such as fungi. To reduce this knowledge deficit, we present the first large-scale metaproteomic study to define the taxonomic composition and potential functional processes of the FGT mycobiome in South African reproductive-age women. We examined FGT fungal communities present in 123 women by collecting lateral vaginal wall swabs for liquid chromatography-tandem mass spectrometry.

2025-03-28 | PXD046053 | Pride

Fungal mycobiome drives IL33 secretion and type 2 immunity in pancreatic cancer

Project description:TH2 and innate lymphoid cells 2 (ILC2) can stimulate tumor growth by secreting pro-tumorigenic cytokines such as IL4, IL5 and IL13. However, the mechanisms by which type 2 immune cells traffic to the tumor microenvironment (TME) are unknown. Here, in pancreatic ductal adenocarcinoma (PDAC), we show that oncogenic KrasG12D (Kras*) increases the expression of IL33 in cancer cells, which upon secretion recruits and activates the TH2 and ILC2. Correspondingly, cancer cell-specific deletion of IL33 reduces TH2 and ILC2 recruitment and promotes tumor regression. Unexpectedly, we discovered that the cellular release of IL33 into the TME is dependent on the intratumoral fungal mycobiome. Genetic deletion of IL33 or anti-fungal treatment decreases TH2 and ILC2 infiltration and increases survival. Consistent with these murine data, high IL33 expression is observed in approximately 20% of human PDAC, and expression is mainly restricted to cancer cells. These data expand our knowledge of the mechanisms driving PDAC tumor progression and identifies therapeutically targetable pathways involving intratumoral mycobiome-driven secretion of IL33.

2022-03-01 | GSE190103 | GEO

Fungal mycobiome drives IL33 secretion and type 2 immunity in pancreatic cancer

Project description:We report the RNAseq of mouse pancreatic cancer cell lines with Kras ON vs Kras OFF. TH2 and innate lymphoid cells 2 (ILC2) can stimulate tumor growth by secreting pro-tumorigenic cytokines such as IL4, IL5 and IL13. However, the mechanisms by which type 2 immune cells traffic to the tumor microenvironment (TME) are unknown. Here, in pancreatic ductal adenocarcinoma (PDAC), we show that oncogenic KrasG12D (Kras*) increases the expression of IL33 in cancer cells, which upon secretion recruits and activates the TH2 and ILC2. Correspondingly, cancer cell-specific deletion of IL33 reduces TH2 and ILC2 recruitment and promotes tumor regression. Unexpectedly, we discovered that the cellular release of IL33 into the TME is dependent on the intratumoral fungal mycobiome. Genetic deletion of IL33 or anti-fungal treatment decreases TH2 and ILC2 infiltration and increases survival. Consistent with these murine data, high IL33 expression is observed in approximately 20% of human PDAC, and expression is mainly restricted to cancer cells. These data expand our knowledge of the mechanisms driving PDAC tumor progression and identifies therapeutically targetable pathways involving intratumoral mycobiome-driven secretion of IL33.

2022-03-01 | GSE190468 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data