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ABSTRACT: Sequencing of common carp (Cyprinus carpio L.) brain

PROVIDER: PRJNA1069873 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27789100_1.fastq.gz	Fastqsanger.gz
	SRR27789100_2.fastq.gz	Fastqsanger.gz
	SRR27789101_1.fastq.gz	Fastqsanger.gz
	SRR27789101_2.fastq.gz	Fastqsanger.gz
	SRR27789087_1.fastq.gz	Fastqsanger.gz

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Identification of miRNA from Cyprinus carpio by high-throughput sequencing and bioinformatics analysis

Project description:Common carp is one of the main commercial fishes captured and cultured worldwide. Although common carp genome is not finished yet, this study provides a first large scale cloning and characterization of common carp miRNAs and their potential targets. These miRNAs add to the growing database of new miRNA and lay the foundation for further understanding of miRNA function in gene regulation of common carp. We constructed a small RNA library from 17 Cyprinus carpio samples.

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Project description:Ichthyophthirius multifiliis is a ciliated protozoan parasite recognized as one of the most pathogenic diseases of wild and cultured freshwater fish. Fish skin mucus plays a significant role against invading pathogens. However, the protein-based modulation against infection with I. multifiliis, of host fish at this barrier is unknown. The aim of this study was to investigate the modulation of the skin mucus after infection with I. multifiliis using quantitative proteomics to provide insights into the post-transcriptional and post-translational regulation of skin mucus proteins. Thus, we investigated the skin mucus proteome of common carp (Cyprinus carpio) using a shotgun proteomic approach at days 1 and 9 after I. multifiliis exposure.

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