Project description:An EWAS study investigating the associations between DNA cytosine methylation status in blood and human physical performance measures in a cross-sectional setting among senior-aged male and female individuals.
2025-08-13 | GSE305174 | GEO
Project description:Fecal microbiome profiling in senior dogs
Project description:Changes in gene expression during peripheral nerves aging, to the single cell level, are unkown. To explore possible age-related changes during healthy aging of peripheral nerves we compared snRNAseq of sciatic nerves from young adult (2-3 months old), senior adult (15-16 months old), and aged (20-30 month old mice.
Project description:In the present study, we investigated the effect of Lactobacillus lactis on healthspan using Caenorhabditis elegans as a model animal. The healthspan of the adult C. elegans fed the mixture of E. coli OP50 and Lactobacillus lactis NBRC 100913 or NBRC 12007 strains was significantly longer than that of animals fed sole OP50 (control). Transcriptional profiling comparing Lactobacillus lactis- and control-fed animals suggested that genes related to “locomotion” were upregulated by Lactobacillus lactis.
Project description:Varicella-zoster virus (VZV), an alphaherpesvirus, causes chickenpox (varicella) in young children with an annual minimum of 140 million new cases and herpes zoster in senior, a painful and debilitating disease with 3-5‰ incidence. A complex structural transcriptome of VZV, which numerous novel transcripts, transcript isoforms, and unknown splice events are found during cell infection. Circular RNA (circRNA), a newly important component of the transcriptome, is increasing discoveries of circRNA function in mammalian cells. However, VZV encoded circRNA remains unexplored. The code used in this study and extended data are available from the GitHub repository (https://github.com/ShaominYang/VZV_circRNA)
2024-04-27 | GSE252124 | GEO
Project description:fecal microbiota compositions of young and senior canines.
| PRJNA865497 | ENA
Project description:Age-related gut microbiota profiling in senior dogs
Project description:Background: Aging is associated with progressive metabolic and physiological changes that influence health span in humans and may similarly impact dogs. This study aimed to characterize in the gut microbiota and their associations with host metabolism. Client-owned young adult (2-5 years, > 20 kg, n = 50) and senior (≥ 8 years, > 20 kg, n = 56) dogs were prospectively enrolled and fed a standardized diet for four weeks. Results: Multivariate analyses identified distinct age-related metabolic and microbial signatures, likely linked to oxidative stress, inflammation, and alterations in protein metabolism. Senior dogs exhibited lower plasma concentrations of glycine, tyrosine, and methionine, together with higher variability of plasma succinic acid concentrations. Compared with young adult dogs, senior dogs showed higher relative abundances of Collinsella and Streptococcus, lower relative abundances of Agathobacter, which was positively correlated with plasma vitamin E status, and higher fecal isovalerate concentrations. Conclusions: These findings highlight coordinated age-related changes in host metabolism and gut microbiota and underscore the potential of integrated multi-omics approaches to identify biological markers and nutritional targets relevant to canine aging.
Project description:The presence of tagatose in Lactobacillus rhamnosus strain GG caused induction of a large number of genes associated with carbohydrate metabolism including the phosphotransferase system. In addition, these results indicate the tagatose enhanced the growth of Lactobacillus casei 01 and Lactobacillus rhamnosus strain GG and their probiotic activities by activating tagatose-associated PTS networks. Two-condition experiment, Lactobacillus rhamnosus GG with glucose vs. Lactobacillus rhamnosus GG with tagatose. For preparing the total RNA, Lactobacillus rhamnosus GG cells were grown at 37M-BM-0C in prebiotic minimum medium supplemented with 2% glucose or tagatose for 24 h.