Project description:Toxoplasma gondii is a ubiquitous protozoan pathogen able to infect both mammalian and avian hosts. Surprisingly, just three strains appear to account for the majority of isolates from Europe and N. America. To test the hypothesis that strain divergence might be driven by differences between mammalian and avian response to infection, we examine in vitro strain-dependent host responses in a representative avian host, the chicken. Chicken embryonic fibroblasts were cultivated in vitro and infected with different strains of Toxoplasma gondii (Type II = ME49, Type III = CEP); host transcriptional responses were then analyzed at 24 hours post-infection.
Project description:Expression profile microarray of human foreskin fibroblast cell comparing control untreated HFF cell with HFF cell infected with ME49 strain.Study on Toxoplasma gondii infection of HFF cell LncRNAs expression, for further studies on the differential exprssion of LncRNAs in HFF cell against the infection of Toxoplasma gondii research provide the basic function.
Project description:Analysis of mRNA-seq reveals difference in gene expression profiles of wild type iBMDM, IRF3-/- iBMDM and IRF7-/- iBMDM infected with ME49, a type II Toxoplasma gondii
Project description:Purpose: Porcine alveolar macrophage was infected by T. gondii including Rh strain and Me49 strain. We want to explore the change of miRNAs after infected with T. gondii in porcine alveolar macrophages. Results: Our study generated six mi RNA expression profiles from macrophages which infect with Rh strain and Me49 and control group in different time. Compare with T. gondii-infected and uninfected with T. gondii, 81 differentially expressed mi RNAs were identified, including 36 novel mi RNAs and 45 mature mi RNAs.
Project description:The mice were acutely and chronically infected with Toxoplasma gondii oocysts, and the mouse brain tissue was obtained, and the total protein was extracted, proteolysed, and enriched for acetylated polypeptides.
Project description:To investigate the transcriptional effects of TgAP2XII-5 on type II Toxoplasma gondii (ME49) in the tachyzoite and bradyzoite stages. Also, to study the transcriptional role of TgAIP1 on ME49 tachyzoites.
Project description:investigate the proximal proteins on the porositophorous vacuole membranes when toxoplasma gondii ME49 infected mouse bone marrow derived dendritic cells were primed under different conditions. The priming condition using IFN gamma will induce the formation of GBP+ puncta on a portion of PVM. The constituents of the GBP+ puncta are also investigated.
Project description:RNA-seq experiment examining the transcriptional profiles of FACS-sorted wild-type and STAT1-deficient microglia isolated from mice infected with the Me49 strain of T. gondii.