Project description:For transcriptomic analysis under drought, re-watering (RW), and re-watering with nitrate (RWN) conditions, wheat seedlings were germinated and transplanted into soil tanks once the first leaf emerged from the coleoptile, then irrigated with 2 L of wheat growth medium containing 5 mM nitrate, after which no further water was added. When soil moisture fell below 5%, seedlings were subjected to drought for one day before being re-watered with 2 L of medium containing either 0 mM (RW) or 5 mM (RWN) nitrate per tank. Leaf samples were collected from wheat seedlings during well-watered, drought, and at 3 hours, 1 day, and 5 days post-RW/RWN, and RNA was extracted for subsequent RNA-Seq analysis.
Project description:One week old bread wheat plantlets were artifically infected with Puccinia triticinae (the causal organism of wheat leaf rust) and samples were collected after one week from infection. Samples were collected after one week from infection, non infected as well. Two loacl varities were used MISR 1 and GEMMZA 7.
2014-07-20 | GSE55297 | GEO
Project description:BSR-seq for LsOFP6a leaf angle
Project description:TaGPC1 and TaGPC2 are NAC-domain transcription factors which accelerate the onset of senescence and facilitate nutrient translocation in wheat. We developed knockout mutants of these genes in tetraploid wheat and used RNA-seq to identify the effect of these mutations on the wheat flag leaf transcriptome during monocarpic senescence. Several transporter-related genes were identified which were upregulated during senescence and differentially expressed between genotypes.
Project description:We performed ChIP-seq for the meiotic strand exchange protein DMC1, which marks an early stage in the meiotic recombination pathway, and the chromosome axis protein ASY1, which promotes interhomolog synapsis and recombination in plants, using tissue collected from immature pre-emergence spikes from wild type bread wheat cultivar Chinese Spring plants. To investigate connections between meiotic recombination and chromatin states in wheat, we also performed ChIP-seq for euchromatic (H3K4me3) and constitutive heterochromatic (H3K9me2 and H3K27me1) marks, and mapped genome-wide nucleosome occupancy via micrococcal nuclease sequencing (MNase-seq) using leaf tissue from Chinese Spring.
