Project description:We have used a strain of Tobacco etch potyvirus (TEV) experimentally adapted to Arabidopsis thaliana ecotype Ler-0 to infect a set of seven A. thaliana plant ecotypes(Col-0, Ei-2, Wt-1, ler-0, Oy-0, St-0). Each ecotype was inoculated with the same amount of the virus. Using commercial microarrays containing probes Arabidopsis thaliana ssp. Col-0 plant transcripts, we explored the effect of viral infection in the plant transcriptome
Project description:his study investigates how Arabidopsis thaliana seeds integrate genetic and environmental stress during development. We analyzed wild-type (WT) and cruabc triple seed-storage-protein (SSP) mutants to test whether proteome rebalancing—the ability to maintain overall amino acid and protein composition despite loss of SSPs—persists under additional stress. Plants were subjected to low nitrogen, high nitrogen, or drought treatments applied exclusively during seed filling. We measured seed physiological traits, carbon and nitrogen content, fatty-acid composition, free and protein-bound amino acids, and performed DIA-based total proteomics.
