Project description:The goal of this study is to compare NGS-derived wild-type Raw264.7 transcriptome profiling (RNA-seq) to NGS-derived Fibrillarin knockdown (Fbl+/-) Raw264.7 RNA-seq. The mRNA profiles of wild-type Raw264.7 and Fbl+/− Raw264.7 were generated by deep sequencing, in duplicate, using illumina NovaSeq 6000.
Project description:The goal of this study is to compare transcriptome-wide Nm-seq on the poly A+ RNA of wild-type Raw264.7 macrophages to transcriptome-wide Nm-seq on the poly A+ RNA of Raw264.7 macrophages after VSV infection . The Nm-seq profiles of wild-type Raw264.7 poly A+ RNA and VSV infected Raw264.7 poly A+ RNA were generated by deep sequencing using Illumina HiSeq4000 sequencer.
Project description:to discover the miRNAs involved in the production of cytokines by RAW264.7 cells treated with LPS and ginsinoside Rd monomer We then performed gene expression profiling analysis using data obtained from RNA-seq of RAW264.7 cells.
Project description:We performed transcriptome analyses of miR-26a-overexpressing RAW264.7 cells (RAW264.7.miR-26a.OE) and NC-plasmid-transfected RAW264.7 cells (RAW264.7.NC), using RNA-seq . We identified 121 genes that were downregulated in RAW264.7.miR-26a.OE cells compared with RAW264.7.NC cells. Among the downregulated genes, we identified four putative target genes, using TargetScan (www.targetscan.org), including EphA2, Map3k9, Nhsl1, and Otud1, which all contain a putative miR-26a seed sequence.
Project description:To investigate the effects of 25-HC on the polarization of macrophage RAW264.7,RAW264.7 cells treated with 25-HC and untreated control were collected for RNA sequencing We then performed gene expression profiling analysis using data obtained from RNA-seq of RAW264.7 cell treated with 25-HC and untreated control
Project description:In this study, we employed a combination of Endosome RNA-seq and iCLIP-seq technologies to identify long non-coding RNA associated with TLR7 and Myd88 in mouse HA-mTLR7 stable overexpression and wild type RAW264.7 cells. RNA-seqs of wild type and Lnc-Atg16l1 RAW264.7 cells were applied to function study. We performed icSHAPE-seq to investigate RNA secondary structure of Lnc-Atg16l1 in RAW264.7 cells with or without stumulatied by R837, as well as in spleen cells from BXSB.Yaa and BXSB.B6 mice.
Project description:The goal of this study is to compare transcriptome-wide Nm-seq on the poly A+ RNA of wild-type Raw264.7 macrophages to transcriptome-wide Nm-seq on the poly A+ RNA of Fbl+/- Raw264.7 macrophages . The Nm-seq profiles were generated by deep sequencing using Illumina HiSeq4000 sequencer.