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ABSTRACT: RNA-seq of RAW264.7 cells

PROVIDER: PRJNA723362 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR14289415_1.fastq.gz	Fastqsanger.gz
	SRR14289415_2.fastq.gz	Fastqsanger.gz
	SRR14289416_1.fastq.gz	Fastqsanger.gz
	SRR14289416_2.fastq.gz	Fastqsanger.gz
	SRR14289417_1.fastq.gz	Fastqsanger.gz

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Project description:RAW264.7 RNA-seq

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Ginsenoside Rd monomer alleviates ulcerative colitis by regulating miRNAs and target genes in macrophages

Project description:to discover the miRNAs involved in the production of cytokines by RAW264.7 cells treated with LPS and ginsinoside Rd monomer We then performed gene expression profiling analysis using data obtained from RNA-seq of RAW264.7 cells.

2024-04-01 | GSE231990 | GEO

MiR-26a targets EphA2 to resist intracellular Listeria monocytogenes in macrophages

Project description:We performed transcriptome analyses of miR-26a-overexpressing RAW264.7 cells (RAW264.7.miR-26a.OE) and NC-plasmid-transfected RAW264.7 cells (RAW264.7.NC), using RNA-seq . We identified 121 genes that were downregulated in RAW264.7.miR-26a.OE cells compared with RAW264.7.NC cells. Among the downregulated genes, we identified four putative target genes, using TargetScan (www.targetscan.org), including EphA2, Map3k9, Nhsl1, and Otud1, which all contain a putative miR-26a seed sequence.

2022-06-01 | GSE149842 | GEO

Promotion of TLR7-MyD88-dependent Inflammation and Autoimmunity through Stem-loop Changes in Lnc-Atg16l1

Project description:In this study, we employed a combination of Endosome RNA-seq and iCLIP-seq technologies to identify long non-coding RNA associated with TLR7 and Myd88 in mouse HA-mTLR7 stable overexpression and wild type RAW264.7 cells. RNA-seqs of wild type and Lnc-Atg16l1 RAW264.7 cells were applied to function study. We performed icSHAPE-seq to investigate RNA secondary structure of Lnc-Atg16l1 in RAW264.7 cells with or without stumulatied by R837, as well as in spleen cells from BXSB.Yaa and BXSB.B6 mice.

2024-10-12 | GSE228970 | GEO

Project description:RNA-Seq of RAW264.7

| PRJNA1072542 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and Fbl+/- Raw264.7 Transcriptomes

Project description:The goal of this study is to compare NGS-derived wild-type Raw264.7 transcriptome profiling (RNA-seq) to NGS-derived Fibrillarin knockdown (Fbl+/-) Raw264.7 RNA-seq. The mRNA profiles of wild-type Raw264.7 and Fbl+/− Raw264.7 were generated by deep sequencing, in duplicate, using illumina NovaSeq 6000.

2022-04-05 | GSE185660 | GEO

Nm-seq finds modified 2’-O-methylation sites in mRNA of Raw264.7

Project description:The goal of this study is to compare transcriptome-wide Nm-seq on the poly A+ RNA of wild-type Raw264.7 macrophages to transcriptome-wide Nm-seq on the poly A+ RNA of Raw264.7 macrophages after VSV infection . The Nm-seq profiles of wild-type Raw264.7 poly A+ RNA and VSV infected Raw264.7 poly A+ RNA were generated by deep sequencing using Illumina HiSeq4000 sequencer.

2022-04-05 | GSE185661 | GEO

Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [vehicle and 25-HC]

Project description:To investigate the effects of 25-HC on the polarization of macrophage RAW264.7,RAW264.7 cells treated with 25-HC and untreated control were collected for RNA sequencing We then performed gene expression profiling analysis using data obtained from RNA-seq of RAW264.7 cell treated with 25-HC and untreated control

2024-08-31 | GSE222623 | GEO

Expression profiles of M2-polarized RAW264.7 cells [RAW_M2vsM0_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of M2-polarized RAW264.7 cells compared with M0 RAW264.7 cells. Methods: RAW264.7 cells were polarized toward M2 using IL-4. M0 RAW264.7 cells were maintained in culture without IL-4. Total RNA of M2 and M0 RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (M2 versus M0 RAW264.7 cells) with three samples each. Results: There were significant differences between M2 and M0 RAW264.7 cells. Conclusions: Polarization of RAW264.7 cells from M0 to M2 induces various changes at the transcription level.

2024-03-31 | GSE183078 | GEO

Expression profiles of M1-polarized RAW264.7 cells [RAW_M1vsM0_RNA-seq]

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to evaluate the gene expression of M1-polarized RAW264.7 cells compared with M0 RAW264.7 cells. Methods: RAW264.7 cells were polarized toward M1 using LPS and IFN-γ. M0 RAW264.7 cells were maintained in culture without LPS and IFN-γ. Total RNA of M1 and M0 RAW264.7 cells was extracted. RAW264.7 cells RNA profiles were generated by deep sequencing for two groups (M1 versus M0 RAW264.7 cells) with three samples each. Results: There were significant differences between M1 and M0 RAW264.7 cells. Conclusions: Polarization of RAW264.7 cells from M0 to M1 induces various changes at the transcription level.

2024-03-31 | GSE183077 | GEO

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