Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: RNA seq of Human Peripheral Blood Mononuclear Cells of Thalassemia subjects

PROVIDER: PRJNA1074075 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28013967_1.fastq.gz	Fastqsanger.gz
	SRR28013967_2.fastq.gz	Fastqsanger.gz
	SRR28013968_1.fastq.gz	Fastqsanger.gz
	SRR28013968_2.fastq.gz	Fastqsanger.gz
	SRR28013969_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Homo sapiens

Project description:RNA-seq of human peripheral blood mononuclear cells

| PRJNA343985 | ENA

Homo sapiens

Project description:RBC RNA Seq of Thalassemia subjects

| PRJNA1074078 | ENA

Homo sapiens

Project description:The DNA methylome of human peripheral blood mononuclear cells

| PRJNA119949 | ENA

Homo sapiens

Project description:CD6 co-stimulation of human peripheral blood mononuclear cells

| PRJNA115223 | ENA

Homo sapiens

Project description:Transcriptome analysis of human peripheral blood mononuclear cell (PBMC)

| PRJNA422156 | ENA

Homo sapiens

Project description:Ionizing Radiation Induced micro RNA Expression Alterations in Human Peripheral Blood Mononuclear Cells

| PRJNA241428 | ENA

Mitochondrial proteome of human leuekmia.

Project description:Proteome characterization of isolated mitochondria samples prepared from three commonly used acute leukemia cell lines (HL-60, KG-1, MV-4-11). Data were compared to isolated mitochondria prepared from peripheral blood mononuclear cells (PBMC), isolated from healthy human subjects.

2020-08-04 | PXD020715 | JPOST Repository

Homo sapiens

Project description:RNA-Seq profiling of 29 immune cell types and peripheral blood mononuclear cells

| PRJNA418002 | ENA

Homo sapiens

Project description:RNA-Seq of peripheral blood mononuclear cells in asthmatic and normal surgical populations

| PRJNA1188800 | ENA

Selective inhibition of Homo sapiens M1 macrophages where SP140 mRNA is inhibited using siRNA

Project description:As part of our study in understanding the role of SP140 in inflammatory pathways in macrophages, we inhibited SP140 mRNA using siRNA. Peripheral blood mononuclear cells (PBMCs) were obtained from whole blood of healthy donors (from Sanquin Institute Amsterdam or from GSK Stevenage Blood Donation Unit) by Ficoll density gradient (Invitrogen). CD14+ monocytes were positively selected from PBMCs using CD14 Microbeads according to the manufacturer’s instructions (Miltenyi Biotec). CD14+ cells were differentiated with 20 ng/mL of macrophage colony-stimulating factor (M-CSF) (R&D systems) for 3 days followed by 3 days of polarization into classically activated (inflammatory) M1 macrophages (100 ng/mL IFN-γ; R&D systems). M1 macrophages were transfected with siGENOME human smartpool SP140 siRNA or non-targeting scrambled siRNA for 48h with DharmaFECT™ transfection reagents according to manufacturer’s protocol (Dharmacon). The cells were left unstimulated or stimulated with 100 ng/mL LPS (E. coli 0111:B4; Sigma) for 4h (for qPCR) or 24h (for Elisa). The cells were lysed (ISOLATE II RNA Lysis Buffer RLY-Bioline) for RNA extraction.150 ng total RNA was labelled using the cRNA labelling kit for Illumina BeadArrays (Ambion) and hybridized with Ref8v3 BeadArrays (Illumina). Arrays were scanned on a BeadArray 500GX scanner and data were normalized using quantile normalization with background subtraction (GenomeStudio software; Illumina). This submission only contains processed data

2022-07-22 | E-MTAB-12011 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data