Project description:The potato cyst nematodes (PCN), Globodera pallida and Globodera rostochiensis, are important parasites of potato. PCN have complex, biotrophic interactions with their hosts that involve gene expression changes in both the nematode and the host plant. The aim of this study is to determine key genes that are differentially expressed in Globodera pallida life cycle stages and during the initiation of the feeding site in susceptible or partially resistant potato genotypes. For this purpose, two microarray experiments were designed: i) a comparison of eggs, infective second stage juveniles (J2) and sedentary parasitic stage J2s (SJ2); ii) a comparison of SJ2 at eight days after inoculation (DAI) in the susceptible cultivar (Desiree) and two partially resistant lines. The data set in this submission came from experiment (I). The results show differential expression of G. pallida genes during the stages studied, including previously characterized effectors. Also, a large number of genes changed their expression between the SJ2 in the susceptible cultivar compared to those infecting partially resistant lines; the number of genes with modified expression were smaller when two partially resistant lines were compared. Additionally, a histopathological study was performed at several time points (7, 14 and 30 DAI) showing the similarities between both partially resistant lines with a delay and degeneration in the formation of the syncytia in comparison to the susceptible cultivar. Females at 30 DAI in partially resistant lines showed a delay in their development in comparison to the susceptible cultivar.

2012-04-12 | E-MTAB-999 | biostudies-arrayexpress

JHI Gp 15k host comparison

Project description:The potato cyst nematodes (PCN), Globodera pallida and Globodera rostochiensis, are important parasites of potato. PCN have complex, biotrophic interactions with their hosts that involve gene expression changes in both the nematode and the host plant. The aim of this study is to determine key genes that are differentially expressed in Globodera pallida life cycle stages and during the initiation of the feeding site in susceptible or partially resistant potato genotypes. For this purpose, two microarray experiments were designed: i) a comparison of eggs, infective second stage juveniles (J2) and sedentary parasitic stage J2s (SJ2); ii) a comparison of SJ2 at eight days after inoculation (DAI) in the susceptible cultivar (Desiree) and two partially resistant lines. The data set in this submission came from experiment (ii). (Experiment (I) has also been deposited in ArrayExpress under accession E-MTAB-999). The results show differential expression of G. pallida genes during the stages studied, including previously characterized effectors. Also, a large number of genes changed their expression between the SJ2 in the susceptible cultivar compared to those infecting partially resistant lines; the number of genes with modified expression were smaller when two partially resistant lines were compared. Additionally, a histopathological study was performed at several time points (7, 14 and 30 DAI) showing the similarities between both partially resistant lines with a delay and degeneration in the formation of the syncytia in comparison to the susceptible cultivar. Females at 30 DAI in partially resistant lines showed a delay in their development in comparison to the susceptible cultivar.

2012-04-12 | E-MTAB-1010 | biostudies-arrayexpress

Globodera pallida genomic sequencing

Project description:Globodera pallida genomic sequencing

| PRJEB2242 | ENA

Globodera pallida transcriptomics

Project description:As part of the Globodera pallida (potato cyst nematode) genome project weare profiling the transcriptome of the parasite across its life cycle usingRNA-Seq. . This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2012-11-28 | E-ERAD-55 | biostudies-arrayexpress

RNAseq of gall-enriched tissue of Solanum tuberosum infected by Globodera pallida

Project description:We compared the gene expression of Solanum tuberosum genotype SH infected with either Globodera pallida D383, E400 or mock infected. After we (mock)-infected the plants we collected gall-enriched tissue 3 and 6 days post infection. The transcriptomes were measured using RNA-seq. The aim of the experiment was to determine how the plant immune response developed after activation of the resistance gene Gpa2 by D383, as E400 is able to overcome that resistance.

2022-04-30 | E-MTAB-11646 | biostudies-arrayexpress

Potato cyst nematode repsonses in potato

Project description:Sets of seven 2-week old potato plants carrying the nematode resistance gene H1, grown from tuber ‘chits’ in sandy loam at a constant temperature of 20 ºC and a light cycle of 16 hour light/8 hour dark, were each inoculated in the roots evenly with 2000 juveniles of the virulent potato cyst nematode Globodera pallida, or with the avirulent G. rostochiensis pathotype Ro1, or with water. Plants were manually watered throughout the duration of the experiment. 5, 17 and 33 days after inoculation, the roots were carefully washed and root tissue samples were individually flash-frozen in liquid nitrogen and stored at –80 ºC. Total RNA isolations were performed using the Qiagen RNeasy kit. All samples were treated with DNase. The experiment was replicated twice. Keywords: Direct comparison

2007-09-01 | GSE8220 | GEO

Genome assembly of Globodera pallida Rookmaker

Project description:Globodera pallida genome based on the Rookmaker population

| PRJEB91928 | ENA