Project description:ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days). Two biological repeat experiments were conducted and the raw data was analyzed applying Affymetrix GCOS software. Experiment Overall Design: ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days).
Project description:ATH1 GeneChip was used for gene expression analysis of wild-type plants and dor mutant under drought treatment (both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed by completely depriving of irrigation for 10 days). Two biological repeat experiments were conducted and the raw data was analyzed applying Affymetrix GCOS software. Keywords: both the wild-type and dor plants were grown under normal watering conditions for 24 days and then stressed for 10 days
Project description:Crop plants are often exposed to the combination of drought and pathogen stress. Transcriptome studies on Arabidopsis thaliana and other plants unveiled activation of shared molecular defense mechanisms between under individual and combined stresses. These shared plant responses are characterized by commonly regulated genes under individual and combined stresses. Based on the previous studies, G-box binding factor 3 (GBF3) is one of the regulatory components of such shared responses. However, the mechanistic understanding on the role of GBF3 under combined drought and pathogen stress is not yet decoded. Using genetic approaches, we demonstrated Atgbf3 mutant plants are more susceptible under individual and combined drought and Pseudomonas syringae pv. tomato DC3000 stresses as compared to the wild-type plants. We further analyzed the global transcriptome of Atgbf3 mutant under combined stress to identify its downstream targets to further validate the role of AtGBF3 in combined stress. We used microarrays to detail the global transcriptome reprogramming during AtGBF3-mediated regulation of combined stress.
Project description:Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities. This experiment contains FT-ICR-MS measurements for 103 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22 °C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33 °C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33 °C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 pm). For validation, GC-TOF-MS measurements were done for two genotypes (wildtype, double mutant) and two conditions (drought, control) on partially overlapping samples.
Project description:With frequent fluctuations in global climate, plants often experience co-occurring dry-wet cycles and pathogen infection and this combination adversely affects plant survival. In the past, some studies indicated that morpho-physiological responses of plants to the combined stress are different from the individual stressed plants. However, interaction of drought stressed or drought recovered plants with pathogen has not been widely studied at molecular level. Such studies are important to understand the defense pathways that operate as part of combined stress tolerance mechanism. In this study, Arabidopsis plants were exposed to individual drought stress (soil drying at 40% FC, D), Pseudomonas syringae pv tomato DC3000 (PStDC3000), infection and their combination. Plants recovered from drought stress were also exposed to PStDC3000. Beside we have also infiltrated P. syringae pv tabaci (PSta, non-host pathogen) individually or in combination with drought stress. Using Affymetrix WT gene 1.0 ST array, global transcriptome profiling of plants leaves under individual drought stress and pathogen infection was compared with their combination. Results implicate that plants exposed to combined drought and pathogen stress experience a new state of stress where each combination of stressor and their timing defines the plant responses and thus should be studied explicitly. Global transcriptional analysis in Arabidopsis leaves exposed to individual and combined drought and pathogen stress.
Project description:14-3-3 proteins constitute a class of highly conserved regulatory proteins present in all eukaryotic cells. In plants, they are involved in diverse biological processes, including responses to abiotic stresses. Here, we investigated the roles of two 14-3-3 proteins, GRF6 and GRF8, in the drought tolerance of Arabidopsis thaliana through comparative transcriptome profiling of wild-type (WT) and grf6 grf8 mutant plants.
Project description:To further elucidate the translational roles of NCR1 involed in drought stress, we conducted a comparative leaf proteomic analysis of 24-day-old ncr1 mutant and WT plants under well-watered and drought stress conditions.
Project description:To further investigate how HID2 regulates translation during drought responses, we performed a comparative leaf proteome analysis using 24-day-old hid2 mutant and WT plants grown under well-watered and drought stress conditions.