Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA107919 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Efficient and sustained knockdown of DNMT1 transcript and protein was achieved using a consecutive transfection protocol in the mouse pituitary adenoma cell line, AtT-20. Genome wide microarray analysis identified 91 transcripts that were significantly differentially expressed relative to cells treated with a non-targeting control. Keywords: Differential expression AtT-20 cells were repeatedly treated with 20 nM siRNA (siDNMT1 or siNT) over an eight day period. After eight days total RNA was extracted and prepared for microarray analysis. The procedure was repeated three times independently.
2008-12-30 | E-GEOD-10552 | biostudies-arrayexpress
Project description:Efficient and sustained knockdown of DNMT1 transcript and protein was achieved using a consecutive transfection protocol in the mouse pituitary adenoma cell line, AtT-20. Genome wide microarray analysis identified 91 transcripts that were significantly differentially expressed relative to cells treated with a non-targeting control. Keywords: Differential expression
2008-12-31 | GSE10552 | GEO
Project description:RNAseq in Pax7-reprogrammed corticotropes AtT-20 cells
| PRJNA343794 | ENA
Project description:ChIPseq in Pax7-reprogrammed corticotropes AtT-20 cells
| PRJNA343795 | ENA
Project description:ATACseq in Pax7-reprogrammed corticotropes AtT-20 cells
| PRJNA343786 | ENA
Project description:Murine corticotroph pituitary AtT-20 cells: Crh treatment time course
| PRJNA109741 | ENA
Project description:Whole Genome Bisulfite Sequencing in Pax7-reprogrammed corticotropes AtT-20 cells
| PRJNA343792 | ENA
Project description:Genome wide expression profiling to determine the overlap of Affymetrix-signals with SOLID sequencing RNA was extracted using the Qiagen RNeasy kit following the manufacturers guidelines, arrays were prepared and hybridized following the Affymetrix protocol.
2011-01-06 | GSE19767 | GEO
Project description:Introgressed variants from other species can be an important source of genetic variation because they may arise rapidly, can include multiple mutations on a single haplotype, and have often been pretested by selection in the species of origin. Although introgressed alleles are generally deleterious, several studies have reported introgression as the source of adaptive alleles-including the rodenticide-resistant variant of Vkorc1 that introgressed from Mus spretus into European populations of Mus musculus domesticus. Here, we conducted bidirectional genome scans to characterize introgressed regions into one wild population of M. spretus from Spain and three wild populations of M. m. domesticus from France, Germany, and Iran. Despite the fact that these species show considerable intrinsic postzygotic reproductive isolation, introgression was observed in all individuals, including in the M. musculus reference genome (GRCm38). Mus spretus individuals had a greater proportion of introgression compared with M. m. domesticus, and within M. m. domesticus, the proportion of introgression decreased with geographic distance from the area of sympatry. Introgression was observed on all autosomes for both species, but not on the X-chromosome in M. m. domesticus, consistent with known X-linked hybrid sterility and inviability genes that have been mapped to the M. spretus X-chromosome. Tract lengths were generally short with a few outliers of up to 2.7 Mb. Interestingly, the longest introgressed tracts were in olfactory receptor regions, and introgressed tracts were significantly enriched for olfactory receptor genes in both species, suggesting that introgression may be a source of functional novelty even between species with high barriers to gene flow.
| S-EPMC8784167 | biostudies-literature
Project description:30 µg proteins per sample were reduced (20 mM DTT Sigma, room temperature RT, 1 h) and S-alkylated (50 mM IAA Sigma, 1 h, dark). The remaining IAA was quenched with 20 mM DTT for 1 h in the dark. The proteins were digested with 1:50(w/w enzyme:protein) MS-grade trypsin/lys-C mix (Thermo Scientific) for 24 h at RT. The enzyme digestion was quenched by lowering the pH with formic acid (Fisher) and the sample was desalted with ZipTip C18 (Merck-Millipore) column. Samples were resuspended in 10 µl of 0.2% formic acid prior LC-MS/MS analysis.
2025-05-06 | PXD051730 | Pride