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Transcriptional response to varying air humidity in Arabidopsis

ABSTRACT: Transcriptional response to varying air humidity in Arabidopsis

PROVIDER: PRJNA1079275 | ENA |

REPOSITORIES: ENA

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Transcriptional response to varying air humidity in Arabidopsis

Project description:To investigate the molecular mechanism of plant response to air humidity, wild-type plants and pip2;5, a mutant defective in water transport, were subjected 24 hours of high and low air humidity treatments. The results showed that genes related to hypoxia response, ethylene pathways, and ABA responses were differentially expressed in the pip2;5 mutant compared to the wild type.

2025-08-21 | GSE256367 | GEO

Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

Project description:The goal of this study is to demonstrate the global expression profile of Arabidopsis Col-0 wild type plant leaves in response to low, moderate and high humidity.

2023-10-17 | GSE236463 | GEO

Genome-wide expression analysis of Arabidopsis Col-0 plant and clf mutant plant under high humidity [RNA-seq]

Project description:Purpose: The goal of this study is to demonstrate the global expression profile of Arabidopsis wild type and clf mutant plants in response to high air humidity Methods: Four-week-old Arabidopsis plant leaves were treated with ~95% relative humidity for 0.5 hour (0h as control) Results and Conclusions: This study showed that many genes including ABA-responsive genes and stomotal movement-related genes were differentially expressed in the clf mutant plant.

2024-09-06 | GSE183558 | GEO

Genome-wide expression analysis of Arabidopsis Col-0 plants under BTH treatment and high or low humidity

Project description:The goal of this study is to demonstrate the global expression profile of four-week-old Arabidopsis Col-0 wild type plant leaves in response to high or low air humidity, under water or BTH (SA analog) treatment.

2023-10-17 | GSE210893 | GEO

Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

Project description:Genome-wide expression analysis of Arabidopsis Col-0 plants under different air humidity

| PRJNA991427 | ENA

'Microarray analysis of abiotic stress responses in three-week-old Arabidopsis thaliana plants'' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities'

Project description:This experiment contains microarray measurements for 135 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22Â°C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33Â°C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33Â°C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 p.m.).

2017-06-20 | E-MTAB-4867 | biostudies-arrayexpress

Quantitative proteomics and phosphoproteomics analysis of Arabidopsis Col-0 plants under high humidity

Project description:we performed data-independent acquisition (DIA)-based proteomics and phosphoproteomics to reveal high humidity-responsive protein abundance and phosphorylation changes of Arabidopsis membrane proteins at a dynamic time scale (i.e., 0.5 to 24 h after high humidity exposure).

2022-08-19 | PXD036157 |

Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue

Project description:Nontargeted and targeted metabolomics measurements of abiotic stress responses in three-week-old Arabidopsis thaliana plants' rosette leaf tissue for Col-0 wild type plants and double/triple knockout mutants of aquaporins (pip2;1 pip2;2 and pip2;1 pip2;2 pip2;4) treated with drought, heat at different air humidities, or combined drought-heat stress at different air humidities. This experiment contains FT-ICR-MS measurements for 103 Arabidopsis thaliana rosette leaf samples covering three genotypes under six different environmental conditions. The three genotypes comprise the Col-0 wildtype and two loss-of-function mutants of aquaporins, a pip2;1 pip2;2 double mutant and a pip2;1 pip2;2 pip2;4 triple mutant (respective AGI locus identifiers: AT3G53420, AT2G37170, AT5G60660). The six conditions include control condition (well-watered, 22 °C, 70% relative air humidity), drought stress (one week without watering), heat stress without changing the absolute humidity of the ambient air (6 hours at 33 °C, 37% relative air humidity), heat stress with supplemented air humidity to maintain a constant vapor pressure deficit before and during the heat episode (6 hours at 33 °C, 84% relative air humidity), and the combinations of drought pretreatment with each of the two heat stress variants (one week of drought followed by 6 hours of heat stress). Samples from all conditions were harvested at the same time (within 15 min starting at 5 pm). For validation, GC-TOF-MS measurements were done for two genotypes (wildtype, double mutant) and two conditions (drought, control) on partially overlapping samples.

2017-07-17 | MTBLS355 | MetaboLights

Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

Project description:Voice disorders are an important human health condition. Hydration is a commonly recommended preventive measure for voice disorders though it is unclear how vocal fold dehydration, is harmful at the cellular level. Airway surface dehydration can result from exposure to low humidity air. Here we have used a recurring 8-hour low humidity exposure over 15 days to mimic an occupational exposure to a low humidity environment. Exposure to moderate humidity was the control condition. Full thickness soft-tissue samples, including the vocal folds and surrounding laryngeal tissue, were collected for molecular analysis. RT-qPCR demonstrated a significant upregulation of MUC4 (mucin 4) and SCL26A9 (chloride channel) and a large fold-change though statistically non-significant upregulation of SCNNA1 (epithelial sodium channel). Proteomic analysis demonstrated differential regulation of proteins clustering into prospective functional groups of muscle structure and function, oxidative stress response, and the protein chaperonin stress response. Together, the data demonstrate that recurring exposure to low humidity is sufficient to induce both transcriptional and translational level changes in laryngeal tissue and suggest that low humidity exposure induces cellular stress at the level of the vocal folds.

2022-03-29 | MSV000089151 | MassIVE

Recurring exposure to low humidity induces transcriptional and protein level changes in the vocal folds of rabbits

2020-12-07 | MSV000086568 | MassIVE