Project description:Plant propagation requires adventitious root production, and the inability to root cuttings prevents some tree species from being widely used and propagated on a big scale. Studies on the differences in adventitious root production ability between species that are easily rooted and those that are difficult to root are few, despite the fact that many studies have been done to examine the developmental mechanisms of adventitious roots. To attempt to address this problem, we examined the transcriptomes of Populus davidiana (difficult-to-root) and Populus yunnanensis (easy-to-root) spikes at various intervals following cutting. We discovered that the number of differential genes was significantly greater in the 96- and 120-hour groups than in the 0- and 48-hour groups. The majority of growth hormone and cytokinin-related genes were up-regulated in P. yunnanensis, while they were down-regulated in P. davidiana.
Project description:Intervention type:DRUG. Intervention1:Huaier, Dose form:GRANULES, Route of administration:ORAL, intended dose regimen:20 to 60/day by either bulk or split for 3 months to extended term if necessary. Control intervention1:None.
Primary outcome(s): For mRNA libraries, focus on mRNA studies. Data analysis includes sequencing data processing and basic sequencing data quality control, prediction of new transcripts, differential expression analysis of genes. Gene Ontology (GO) and the KEGG pathway database are used for annotation and enrichment analysis of up-regulated genes and down-regulated genes.
For small RNA libraries, data analysis includes sequencing data process and sequencing data process QC, small RNA distribution across the genome, rRNA, tRNA, alignment with snRNA and snoRNA, construction of known miRNA expression pattern, prediction New miRNA and Study of their secondary structure Based on the expression pattern of miRNA, we perform not only GO / KEGG annotation and enrichment, but also different expression analysis.. Timepoint:RNA sequencing of 240 blood samples of 80 cases and its analysis, scheduled from June 30, 2022..