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Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

ABSTRACT: Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

PROVIDER: PRJNA1084726 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28237408_1.fastq.gz	Fastqsanger.gz
	SRR28237408_2.fastq.gz	Fastqsanger.gz
	SRR28237409_1.fastq.gz	Fastqsanger.gz
	SRR28237409_2.fastq.gz	Fastqsanger.gz
	SRR28237410_1.fastq.gz	Fastqsanger.gz

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Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 4 hours post-treatment

2025-04-23 | GSE260955 | GEO

bulk RNA sequencing for GL261 cells treated with propionate

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 24 hours post-treatment

2025-04-23 | GSE233412 | GEO

bulk RNA sequencing for GL261 cells treated with propionate

Project description:bulk RNA sequencing for GL261 cells treated with propionate

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Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:GL261 cells are one of the most common mouse glioblastoma cells used for in vivo studies in a immunocompetent model. We analyze GL261 and stem cells derived from them (GL261-GSCs) using scRNA-Seq.

2024-03-28 | GSE244301 | GEO

The effect of antibiotics and propionate treatment on astrocyte transcription in APPPS1-21 mice

Project description:Previously, we have shown that broad spectrum antibiotic treatment reduces reactive astrocyte phenotypes in the APPPS1-21 model of AD-related amyloidosis. We have also found that antibiotics selectively increases propionate levels and exogenous propionate treatment recapitulates phenotypes observed in antibiotic treated mice. In the current study, we wanted to assess astrocyte transcriptional state using bulk RNA sequencing. To accomplish this we used translating ribosome affinity purification (TRAP) sequencing, a ribosomal protein L10a is fused to eGFP under the control of a cell type specific promoter in a transgenic mouse model. We crossed APPPS1-21 mice to the Aldh1l1-eGFP/Rpl10a bacTRAP mouse model and progeny were treated with antibiotics, propionate, or VHL and performed bulk TRAPseq.

2025-05-01 | GSE295458 | GEO

Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:GL261 cells are one of the most common mouse glioblastoma cells used for in vivo studies in a immunocompetent model.

2024-03-28 | GSE246262 | GEO

Investigation on the anaerobic propionate degradation by Escherichia coli K12

Project description:Propionate is an abundant carboxylic acid in nature. Microorganisms metabolize propionate aerobically via the 2-methylcitrate pathway. This pathway depends on a series of three reactions in the citric acid cycle that leads to the conversion of succinate to oxaloacetate. Interestingly, the gamma-proteobacterium Escherichia coli can use propionate as a carbon and electron source under oxic but not under anoxic conditions. The typical downregulation of the citric acid cycle under anoxic conditions is only partially responsible for the inability to use propionate under anoxic conditions since an arcA mutant shows very limited growth on propionate. RT-PCR and transcriptomic analysis revealed a post-transcriptional regulation of the prp-genecluster encoding the necessary enzymes for propionate metabolism. The polycistronic mRNA was hydrolyzed in the 3`-5` direction under anoxic conditions. This regulatory strategy is highly constructive because the last gene of the operon encodes the first enzyme of the propionate metabolism. Further analysis revealed that RNase R catalyzes the hydrolysis of the prp transcripts. Consequently, an rnr-deletion strain could metabolize propionate under anoxic conditions. To the best of our knowledge, this is the first study describing the influence of RNase R on the anaerobic metabolism of E. coli.

2017-12-14 | GSE78814 | GEO

Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

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Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

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Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation [ChIP-Seq 2]

Project description:Propiogenic substrate catabolism and gut bacteria produce propionate, a post-translational protein modifier. Here, using a mouse model of propionic acidaemia (PA), we characterise how disturbances to propionate metabolism modify histones and affect cardiac gene expression and function. Plasma propionate was raised in PA mice, but male hearts accumulated a smaller propionyl-CoA excess, correlating with -alanine build-up. Raising -alanine experimentally in myocytes cultured under propionate-stress reduced propionyl-CoA and partially reversed the propionate-evoked metabolic disturbance. Female PA hearts manifested a moderate diastolic dysfunction phenotype, with raised diastolic [Ca2+], expanded end-systolic ventricular volume, and reduced stroke volume. Differentially-expressed genes included Pde9a and Mme that relate to contractile dysfunction through down-scaled cGMP signalling, consistent with phosphoproteome changes. Propionate was traced to histone H3 propionylation and increased acetylation genome-wide, including at Pde9a and Mme promoters, with more pronounced effects in female PA hearts. We link perturbed propionate metabolism to epigenetic changes that impact cardiac function.

2023-09-28 | GSE205837 | GEO

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