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Bulk RNA sequencing for GL261 cells treated with propionate

ABSTRACT: bulk RNA sequencing for GL261 cells treated with propionate

PROVIDER: PRJNA976219 | ENA |

REPOSITORIES: ENA

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bulk RNA sequencing for GL261 cells treated with propionate

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 24 hours post-treatment

2025-04-23 | GSE233412 | GEO

Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

Project description:To identify the effect of propionate on GL261 cells, we treated GL261 cells with sodium chloride or propionate and extracted RNA at 4 hours post-treatment

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Bulk RNA sequencing for GL261 cells treated with propionate (4h post-treatment)

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Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:GL261 cells are one of the most common mouse glioblastoma cells used for in vivo studies in a immunocompetent model. We analyze GL261 and stem cells derived from them (GL261-GSCs) using scRNA-Seq.

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Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

Project description:Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs

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The effect of antibiotics and propionate treatment on astrocyte transcription in APPPS1-21 mice

Project description:Previously, we have shown that broad spectrum antibiotic treatment reduces reactive astrocyte phenotypes in the APPPS1-21 model of AD-related amyloidosis. We have also found that antibiotics selectively increases propionate levels and exogenous propionate treatment recapitulates phenotypes observed in antibiotic treated mice. In the current study, we wanted to assess astrocyte transcriptional state using bulk RNA sequencing. To accomplish this we used translating ribosome affinity purification (TRAP) sequencing, a ribosomal protein L10a is fused to eGFP under the control of a cell type specific promoter in a transgenic mouse model. We crossed APPPS1-21 mice to the Aldh1l1-eGFP/Rpl10a bacTRAP mouse model and progeny were treated with antibiotics, propionate, or VHL and performed bulk TRAPseq.

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bulk RNA seq data from radiotherapy-treated glioma-bearing mice

Project description:The experiment goal was to unveil any modulation of the tumor micro-environment in GL261 and SB28 bearing mice following radiation treatment.

2025-01-22 | E-MTAB-14751 | biostudies-arrayexpress

single cell-RNA sequencing for glioma cells and immune cells from mouse brain tumor following propionate supplementation

Project description:To identify the effect of propionate on brain tumor microenvironment, we isolated immune cells and brain tumor cells of brain from mice supplemented with sodium chloride as control or sodium propionate in drinking water

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