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Physical Phase Transition of Mineralocorticoid Receptors Determines Preservation Quality of Donor Hearts for Transplantation

ABSTRACT: Physical Phase Transition of Mineralocorticoid Receptors Determines Preservation Quality of Donor Hearts for Transplantation

PROVIDER: PRJNA1085324 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28265061_1.fastq.gz	Fastqsanger.gz
	SRR28265061_2.fastq.gz	Fastqsanger.gz
	SRR28265062_1.fastq.gz	Fastqsanger.gz
	SRR28265062_2.fastq.gz	Fastqsanger.gz
	SRR28265063_1.fastq.gz	Fastqsanger.gz

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Physical Phase Transition of Mineralocorticoid Receptors Determines Preservation Quality of Donor Hearts for Transplantation

Project description:We examine the single nuclear RNA profile in the human donor heart at baseline and 10 hours of storage in HTK with/without canrenone. We also analyze the genomic occupancy of MR in these conditions.

2025-04-03 | GSE261124 | GEO

ChIP-nexus of mineralocorticoid receptors and glucocorticoid receptors in neuroblastoma cells

Project description:Purpose: To compare the binding profiles of mineralocorticoid and glucocorticoid receptors after corticosterone treatment in neuroblastoma cells. Methods: Tagged glucocorticoid receptors (eGFP-rat GR) and mineralocorticoid receptors (mCherry-rat MR) were transiently transfected into Neuro2A mouse neuroblastoma cells and treated with (i) vehicle, (ii) corticosterone 100 nM for 20 mins and (iii) corticosterone 100 nM for 20 mins followed by media repacement twice and 40 mins further incubation in charcoal-stripped serum media. ChIP-nexus samples were prepared in triplicate for deep sequencing using Illumina NextSeq, as well as control samples taken from cell lysates before immunoprecipitation. Sequence data was analyzed and processed for the identification of enriched binding sites, using MACS2, and for the characteristic staggered ChIP-nexus borders, using the MACE pipeline (Wang et al., 2014). Results: Corticosterone-sensitive glucocorticoid receptor (GR) binding sites and mineralocorticoid receptor (MR) binding sites showed considerable overlap, by MACS2 analysis. GR and MR binding sites were highly similar showing recruitment to the same DNA sites, by MACE analysis (Wang et al., 2014). Further analyses showed GR could tether MR to the DNA by GR in a functional manner such that gene expression could be altered by the presence of tethered MR. Conclusions: MR can be recruited to DNA-binding sites by GR, even in the absence of MR DNA-binding. MR can interact in multiple modes at genomic DNA binding sites.

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ChIP-nexus of mineralocorticoid receptors and glucocorticoid receptors in neuroblastoma cells

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Project description:Normothermic machine perfusion (NMP) after static cold storage is increasingly used for preservation and assessment of human donor livers prior to transplantation. Biliary viability assessment during NMP reduces the risk of post-transplant biliary complications. However, understanding molecular changes in the biliary system during NMP remains incomplete. Here, we performed RNA-seq analysis of donor livers undergoing NMP treatment and compared livers with biliary viability scores that were acceptable for transplantation vs those that were not.

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Mineralocorticoid receptor co-expression effect on ligand-induced glucocorticoid receptor interaction with the genome in human cell line

Project description:Our previous results observed glucocorticoid receptor (GR) and mineralocorticoid receptor (MR) physical and functional interactions. RNAseq study (GSE163506) revealed that MR blunted GR ligand-dependent gene regulation in human cell lines. In the current study, we developed U-2 OS cell line-derived cells constitutively expressing GR or MR plus GR. Then, we compared GR – genome interaction before and after dexamethasone treatment.

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Molecular signature of mineralocorticoid signalling in cardiomyocytes

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