Project description:This experiments aimed at elucidating the hepatic transcriptional responses of the flathead grey mullet Mugil cephalus fed with a diet where conventional fishmeal was increasingly replaced with partially defatted black soldier fly (Hermetia illucens, BSF). This fish species is receiving increasing global attention in the aquaculture industry due to its favourable biological characteristics, including low trophic feeding habits and high adaptability to diverse environmental conditions, as well as its relatively low dietary protein requirements, making it a good candidate for production diversification.
Project description:The black soldier fly, Hermetia illucens, is a promising source for sustainable production of proteins, lipids and bioactive substances. Feeding on a variety of decomposing organic substrates which are typically inhabited by a range of different microorganisms like bacteria and fungi, H. illucens larvae is expected to be able to strongly produce antimicrobial peptides (AMPs) and other substances possessing antibiotic activity. It has been shown that H. illucens larvae extract exhibits antimicrobial activity against some microorganisms, such as Escherichia coli and Staphylococcus aureus, and antimicrobial effect on bacterial biofilm. Numbers of AMPs produced by H. illucens larvae have also been identified so far. Moreover, H. illucens larvae extract was recently observed with significant induction of cell envelop stress response (CESR) in B. subtilis, indicative of potential production of antimicrobial compounds. B. subtilis as a well-established Gram‑positive model strain is widely used in scientific research and an arguably robust platform to investigate antimicrobial compounds at the respect of, such as the mode of reaction of and the stress response elicited by corresponding compounds. In this study, we aimed to characterize the stress responses in B. subtilis induced by H. illucens larvae extract by employing transcriptomic study via RNA sequencing technique. With transcriptional profiling, we are expecting to be able to give some hints of potential antimicrobial compounds produced by H. illucens larvae by referring to the transcriptomic researches on well-studied antimicrobial substances.
Project description:Hermetia illucens larvae microbiome Targeted loci environmental
| PRJNA1182311 | ENA
Project description:Diet Fermentation Leads to Microbial Adaptation in Black Soldier Fly (Hermetia illucens Linnaeus, 1758) Larvae Reared on Palm Oil Side Streams
