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The mRNA-binding protein KSRP limits the inflammatory response of macrophages

ABSTRACT: The mRNA-binding protein KSRP limits the inflammatory response of macrophages

PROVIDER: PRJNA1086955 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28320661.fastq.gz	Fastqsanger.gz
	SRR28320662.fastq.gz	Fastqsanger.gz
	SRR28320663.fastq.gz	Fastqsanger.gz
	SRR28320664.fastq.gz	Fastqsanger.gz
	SRR28320665.fastq.gz	Fastqsanger.gz

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Similar Datasets

The mRNA-binding protein KSRP limits the inflammatory response of macrophages

Project description:KH-type splicing regulatory protein (KSRP) is a single-stranded nucleic acid-binding protein with multiple functions. It is known to bind AU-rich motifs within the 3´-untranslated region of mRNA species, which in many cases encode dynamically regulated proteins like cytokines. In the present study, we investigated the role of KSRP for the immunophenotype of macrophages using bone marrow-derived macrophages (BMDM) from wild type (WT) and KSRP-/- mice. RNA sequencing revealed that KSRP-/- BMDM displayed significantly higher mRNA expression levels of genes in-volved in inflammatory and immune responses, particularly type I interferon responses, following LPS stimulation. In line, time kinetics studies revealed increased levels of interferon-γ (IFN-γ), in-terleukin (IL)-1β and IL-6 mRNA in KSRP-/- macrophages after 6 h subsequent to LPS stimulation as compared to WT cultures. On protein level KSRP-/- BMDM displayed higher levels of these cy-tokines after overnight stimulation. Matching results were observed for primary peritoneal mac-rophages of KSRP−/− mice. These showed higher IL-6, Tumor necrosis factor-α (TNF-α), C-X-C mo-tif chemokine 1 (CXCL1) and CC-chemokine ligand 5 (CCL5) protein levels in response to LPS stimulation than the WT controls. As macrophages play a key role in sepsis, the in vivo relevance of KSRP deficiency for cytokine/chemokine production was analyzed in an acute inflammation model. In agreement with our in vitro findings, KSRP-deficient animals showed higher cytokine production upon LPS administration in comparison to WT mice. Taken together, these findings demonstrate that KSRP constitutes as an important negative regulator of cytokine expression in macrophages

2024-04-12 | GSE261444 | GEO

The mRNA-Binding Protein KSRP Limits the Inflammatory Response of Macrophages.

Project description:KH-type splicing regulatory protein (KSRP) is a single-stranded nucleic acid-binding protein with multiple functions. It is known to bind AU-rich motifs within the 3'-untranslated region of mRNA species, which in many cases encode dynamically regulated proteins like cytokines. In the present study, we investigated the role of KSRP for the immunophenotype of macrophages using bone marrow-derived macrophages (BMDM) from wild-type (WT) and KSRP-/- mice. RNA sequencing revealed that KSRP-/- BMDM displayed significantly higher mRNA expression levels of genes involved in inflammatory and immune responses, particularly type I interferon responses, following LPS stimulation. In line, time kinetics studies revealed increased levels of interferon-γ (IFN-γ), interleukin (IL)-1β and IL-6 mRNA in KSRP-/- macrophages after 6 h subsequent to LPS stimulation as compared to WT cultures. At the protein level, KSRP-/- BMDM displayed higher levels of these cytokines after overnight stimulation. Matching results were observed for primary peritoneal macrophages of KSRP-/- mice. These showed higher IL-6, tumor necrosis factor-α (TNF-α), C-X-C motif chemokine 1 (CXCL1) and CC-chemokine ligand 5 (CCL5) protein levels in response to LPS stimulation than the WT controls. As macrophages play a key role in sepsis, the in vivo relevance of KSRP deficiency for cytokine/chemokine production was analyzed in an acute inflammation model. In agreement with our in vitro findings, KSRP-deficient animals showed higher cytokine production upon LPS administration in comparison to WT mice. Taken together, these findings demonstrate that KSRP constitutes an important negative regulator of cytokine expression in macrophages.

| S-EPMC11011855 | biostudies-literature

Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses

Project description:This SuperSeries is composed of the following subset Series: GSE36241: Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses (ChIP-Seq) GSE37051: Identification of a FOXO3/IRF7 circuit that limits inflammatory sequelae of antiviral responses (expression) Refer to individual Series

2012-09-19 | E-GEOD-37052 | biostudies-arrayexpress

Butyrate limits inflammatory macrophage niche in NASH

Project description:Butyrate limits inflammatory macrophage niche in NASH

| PRJNA907018 | ENA

Fatty acid-binding protein 5 limits the anti-inflammatory response in murine macrophages.

Project description:The beginning stages of liver damage induced by various etiologies (i.e. high fat diet, alcohol consumption, toxin exposure) are characterized by abnormal accumulation of lipid in liver. Alterations in intracellular lipid transport, storage, and metabolism accompanied by cellular insult within the liver play an important role in the pathogenesis of liver disease, often involving a sustained inflammatory response. The intracellular lipid transporter, fatty acid binding protein 5 (FABP5), is highly expressed in macrophages and may play an important role in the hepatic inflammatory response after endotoxin exposure in mice. This study tested the hypothesis that FABP5 regulates macrophage response to LPS in male C57bl/6 (wild type) and FABP5 knockout mice, both in vitro and in vivo. Treatment with LPS revealed that loss of FABP5 enhances the number of hepatic F4/80(+) macrophages in the liver despite limited liver injury. Conversely, FABP5 knock out mice display higher mRNA levels of anti-inflammatory cytokines IL-10, arginase, YM-1, and Fizz-1 in liver compared to wild type mice. Bone marrow derived macrophages stimulated with inflammatory (LPS and IFN-γ) or anti-inflammatory (IL-4) mediators also showed significantly higher expression of anti-inflammatory/regulatory factors. These findings reveal a regulatory role of FABP5 in the acute inflammatory response to LPS-induced liver injury, which is consistent with the principle finding that FABP5 is a regulator of macrophage phenotype. Specifically, these findings demonstrate that loss of FABP5 promotes a more anti-inflammatory response.

| S-EPMC4565774 | biostudies-literature

RNA binding protein KSRP mediated miRNAs processing reciprocally regulates granulocytic and monocytic differentiation

Project description:KSRP is involved in RNA processing at multiple levels including alternative splicing, mRNA stability, mRNA localization and translation efficiency. Dysregulation KSRP can cause to hematological malignancies. However, the direct contribution of KSRP to myeloid lineage differentiation is poorly understood. KSRP, as a KH-type splicing regulatory protein, which interacts with single-stand AU-rich element containing mRNA and mediated mRNA decay, also serves as a component of Drosha and Dicer complexes and regulates the biogenesis of miRNAs. Whether KSRP mediated miRNAs maturation participated in myeloid lineage differentiation is unknown. Here, we analysis the miRNAs processing during over-expression KSRP in THP-1 cells by poly-A(riched) RNA-seq and miRNA-Seq. Our results suggest that KSRP regulate a class of miRNAs maturation and have a critical function in myeloid lineage differentiation.

2017-08-01 | GSE87318 | GEO

NLRX1 Limits Inflammatory Neurodegeneration in the Anterior Visual Pathway

Project description:NLRX1 Limits Inflammatory Neurodegeneration in the Anterior Visual Pathway

| PRJNA1126727 | ENA

Butyrate limits inflammatory macrophage niche in NASH [RNA-seq]

Project description:Butyrate limits inflammatory macrophage niche in NASH [RNA-seq]

| PRJNA906470 | ENA

Butyrate limits inflammatory macrophage niche in NASH [ChIP-seq]

Project description:Butyrate limits inflammatory macrophage niche in NASH [ChIP-seq]

| PRJNA907020 | ENA

Gene expression in inflammatory macrophages, tissue resident macrophages and neutrophils

Project description:Genes expression in Ly6C+/F4/80+ inflammatory macrophages, CX3CR1+/F4/80+ tissue resident macrophages and Ly6G+/F4/80- neutrophils which were isolated from day 3 wounds in C57/B6 mice aged 8 weeks by cell sorting Ly6C+ macrophages expressed higher (over 5 folds) levels of 241 genes compared to CX3CR1+ macrophages, and 3382 genes compared to neutrophils

2016-07-01 | E-GEOD-74783 | biostudies-arrayexpress

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