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Gene expression of wild-type C57BL/6 mouse lungs infected with C2-202 human metapneumovirus (HMPV)

ABSTRACT: Gene expression of wild-type C57BL/6 mouse lungs infected with C2-202 human metapneumovirus (HMPV)

PROVIDER: PRJNA1087360 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28335160.fastq.gz	Fastqsanger.gz
	SRR28335161.fastq.gz	Fastqsanger.gz
	SRR28335162.fastq.gz	Fastqsanger.gz
	SRR28335163.fastq.gz	Fastqsanger.gz

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Gene expression of wild-type C57BL/6 mouse lungs infected with C2-202 human metapneumovirus (HMPV)

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Project description:Background. Human Metapneumovirus (HMPV) and Respiratory Syncytial Virus (RSV), are responsible for respiratory diseases mostly in children. In spite of the resemblance between these two pneumoviruses, they elicit a different extent of immune response. miRNAs are small non coding RNAs that regulate gene expression and are involved in numerous cellular processes including the immune system. Methodology. Human monocyte-derived dendritic cells (moDC) were differentiated from peripheral blood mononuclear cells and infected at an MOI of 3 for 24h. RNA was isolated to analyze the miRNAs transcription by high throughput sequencing using illumina technology. Principal findings. Infection with HMPV up-regulated the expression of hsa-miR-4448, while RSV infection induced significant expression of hsa-miR-30a-5p, hsa-miR-182-5p, hsa-miR-1913, hsa37, miR-4448 and hsa-miR-4634. Conclusions/Significance. In human monocyte derived dendritic cells (moDC), RSV and HMPV induced different profiles of miRNA expression. Understanding the changes of miRNA expression profiles by RSV and HMPV in immune cells will further our understanding of the differential immune response induced by these respiratory viruses

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Project description:This experiment is designed to study the effects to HMPV on A549 over time Experiment Overall Design: Confluent monolayers of A549 cells were infected with hMPV at MOI of 1 in serum-free media and harvested at 6, 12, 24, 48, or 72 hours post-infection to extract total RNA using RNAqueousÂ®-Midi Kit (Ambion, Austin, TX), according to manufacturerâs instruction;3 Affymetrix HG-U133 plus 2.0 were used for hybridization.

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