Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human metapneumovirus and mumps virus alter the m6A landscapes in THP-1-derived immature dendritic cells

ABSTRACT: N6-methyladenosine (m6A) is a widespread mRNA modification that regulates RNA metabolism and influences virus-induced immune responses. We performed MeRIP-seq to profile m6A landscapes in THP-1-derived immature dendritic cells (THP-iDCs) before and after infection with Human metapneumovirus (hMPV, NL/00/1) or mumps virus (MuV, JL2). Integration of m6A modifications on key innate immune transcripts with transcriptomic data, together with m6A levels on cellular and viral RNA, revealed a dual role of m6A in viral replication and host immune regulation. These datasets provide a resource for exploring epitranscriptomic regulation during hMPV and MuV infection.

ORGANISM(S): Homo sapiens

PROVIDER: GSE333903 | GEO | 2026/07/10

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

m6A modification landscapes of human metapneumovirus, mumps virus, and host A549 cell RNAs revealed by single-base-resolution GLORI-seq [A549 mRNA]

Project description:We infected human lung adenocarcinoma A549 cells with multiple nonsegmented negative-sense (NNS) RNA viruses, including Human metapneumovirus (hMPV), Mumps virus (MuV), Vesicular stomatitis virus (VSV), and Sendai virus (SeV), and evaluated the differences among these viruses in activating innate immune signaling pathways in epithelial cells through transcriptomic analyses of infected cells. Furthermore, by integrating the effects of viral infection on the m6A landscape of A549 cell transcripts, we analyzed the correlation between m6A epitranscriptomic regulation and the activation of innate immunity–related transcripts, as well as the role of m6A in viral immune evasion.

2026-07-10 | GSE333610 | GEO

m6A modification landscapes of human metapneumovirus, mumps virus, and host A549 cell RNAs revealed by single-base-resolution GLORI-seq [A549 GLORI]

Project description:N6-methyladenosine (m6A) is a common modification on mRNA. Multiple viruses exploit host m6A machinery to modify their own genomic RNA and mRNA, thereby facilitating viral replication and evasion of host innate immune surveillance. In this study, we employed the single-base-resolution high-throughput sequencing technique glyoxal and nitrite-mediated deamination of unmethylated adenosines (GLORI) to identify m6A modification sites on the RNAs of Human metapneumovirus (hMPV, NL/1/00 strain) and Mumps virus (MuV, JL2 strain), as well as to characterize the m6A landscapes of the host cell transcriptome in A549 lung epithelial cells. Extensive m6A modifications on viral RNAs exhibited dynamic and gene-specific distributions, while viral infection significantly reprogrammed the m6A landscape of A549 cells, including increased m6A modification in multiple innate immune signaling pathways. Furthermore, through integrated transcriptomic analyses and modulation of host m6A machinery levels, we demonstrated the dual regulatory roles of m6A in both viral replication and host immune responses.

2026-07-10 | GSE333647 | GEO

Gene expression of wild-type C57BL/6 mouse lungs infected with C2-202 human metapneumovirus (HMPV)

Project description:Human metapneumovirus (HMPV) is a primary causative agent of acute lower respiratory tract infections. We used single cell RNA-sequencing (scRNA-seq) to assess lung immune profiles in a mouse model of HMPV infection.

2024-03-18 | GSE261511 | GEO

Gene expression of wild-type C57BL/6 mouse nasal turbinates infected with C2-202 human metapneumovirus (HMPV)

Project description:Human metapneumovirus (HMPV) is a primary causative agent of acute upper respiratory tract infections. We used single cell RNA-sequencing (scRNA-seq) to assess nasal immune profiles in a mouse model of HMPV infection.

2025-08-25 | GSE305077 | GEO

Structural and molecular properties of inclusion bodies of mumps virus

Project description:Viral RNA synthesis of mononegaviruses occurs in the cytoplasmic membrane-less organelles called as inclusion body (IB). Here, we reported that the IBs of mumps virus (MuV), which is the causative agent of mumps and belongs to the family Paramyxoviridae, had properties of liquid organelles formed by liquid-liquid phase separation. Super-resolution microscopic analysis of the MuV IBs demonstrated that nucleocapsid and phospho-(P) proteins formed the cage-like structure and that viral polymerase was in a reticular pattern and partially co-localized with viral mRNA. In addition, we characterized host RNAs localized in the MuV IBs by a spatial transcriptome analysis and found that G-quadruplex motif sequences containing RNAs (G4-RNAs) were concentrated. In vitro phase separation assay showed that the G4-RNAs interacted with the P protein and enhanced condensation in the P droplets. Taken together, our data show that MuV generates the IBs with a characteristic cage-like structure and host G4-RNAs play an important role in the formation of MuV IBs

2024-10-31 | GSE268004 | GEO

Small hydrophobic protein of human metapneumovirus does not affect virus replication and host gene expression in vitro

Project description:In vitro infection experiment with genetically modified human metapneumovirus (HMPV) to study the function of the viral small hydrophobic (SH) protein.

2014-11-23 | E-MTAB-1152 | biostudies-arrayexpress

Search for host factors located in the vicinity of the cytoplasmic tails of the mumps virus glycoproteins

Project description:Mumps virus (MuV) is the etiological agent of mumps, a disease characterized by painful swelling of the parotid glands and often accompanied by severe complications. To understand the molecular mechanism of MuV infection, a functional analysis of the involved host factors is required. However, little is known about the host factors involved in MuV infection, especially those involved in the late stage of infection. In this project, we introduced the N-terminal and C-terminal fragments of split-TurboID into the cytoplasmic domains of hemagglutinin-neuraminidase and fusion proteins, respectively, and confirmed reconstitution and functional ligase activity of split-TurboID. Following affinity purification and mass spectrometry, 641 proteins were identified as being significantly enriched in the split-TurboID-expressing cells.

2022-10-29 | PXD035151 | Pride

Comparative Analysis Of miRNA Profile In Human Dendritic Cells Infected With Respiratory Syncytial Virus And Human Metapneumovirus

Project description:Background. Human Metapneumovirus (HMPV) and Respiratory Syncytial Virus (RSV), are responsible for respiratory diseases mostly in children. In spite of the resemblance between these two pneumoviruses, they elicit a different extent of immune response. miRNAs are small non coding RNAs that regulate gene expression and are involved in numerous cellular processes including the immune system. Methodology. Human monocyte-derived dendritic cells (moDC) were differentiated from peripheral blood mononuclear cells and infected at an MOI of 3 for 24h. RNA was isolated to analyze the miRNAs transcription by high throughput sequencing using illumina technology. Principal findings. Infection with HMPV up-regulated the expression of hsa-miR-4448, while RSV infection induced significant expression of hsa-miR-30a-5p, hsa-miR-182-5p, hsa-miR-1913, hsa37, miR-4448 and hsa-miR-4634. Conclusions/Significance. In human monocyte derived dendritic cells (moDC), RSV and HMPV induced different profiles of miRNA expression. Understanding the changes of miRNA expression profiles by RSV and HMPV in immune cells will further our understanding of the differential immune response induced by these respiratory viruses

2018-12-10 | GSE111732 | GEO

N6-methyladenosine is a molecular signature for discrimination of self and non-self RNA by cytoplasmic RNA sensor RIG-I

Project description:Internal N6-methyladenosine (m6A) modification of RNA is one of the most common and abundant modifications in eukaryotic cells as well as in viruses. However, the biological role(s) of RNA m6A in virus-host interaction remains elusive. Using human metapneumovirus (hMPV), a medically important non-segmented negative-sense RNA virus as a model, we demonstrate that m6A serves as a molecular marker for innate immune discrimination self and nonself RNAs. We show that hMPV RNAs are m6A methylated and that viral m6A methylation promotes hMPV replication and gene expression. HMPV infection leads to differential expression of interferon-related genes involved in innate immune signaling pathways. Inactivating these m6A sites with synonymous mutations resulted in m6A deficient recombinant hMPVs that induced significantly higher expression of type I interferon that restricted viral replication. Notably, the induction of type I interferons by m6A-deficient rhMPVs and virion RNA was dependent on the cytoplasmic RNA sensor RIG-I, not MDA5. Mechanistically, m6A-deficient virion RNA induces higher expression of RIG-I, enhances its binding affinity to RIG-I, and facilitates the conformational change of RIG-I, leading to enhanced induction of type I IFN expression. The replication of m6A-deficient rhMPVs was attenuated in wild type A459 cells but was restored in cells knocked out for RIG-I and MAVS. Furthermore, m6A-deficient rhMPVs triggered higher type I interferon in vivo and were significantly attenuated in the lower respiratory tract yet retained high immunogenicity in cotton rats. Collectively, our results highlight that (i) virus acquires m6A in their RNAs as a means of mimicking cellular RNA to avoid the detection by innate immunity; and (ii) viral m6A RNA can serve as a novel target to attenuate hMPV for vaccine purposes.

2019-10-18 | GSE136139 | GEO

Mumps orthorubulavirus

Project description:Mumps virus overview

| PRJNA73799 | ENA