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Biosynthesis of oxyresveratrol in mulberry (Morus alba L.) is mediated by a group of p-coumaroyl-CoA 2'-hydroxylases acting upstream of stilbene synthases

ABSTRACT: Biosynthesis of oxyresveratrol in mulberry (Morus alba L.) is mediated by a group of p-coumaroyl-CoA 2'-hydroxylases acting upstream of stilbene synthases

PROVIDER: PRJNA1087683 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28341600_1.fastq.gz	Fastqsanger.gz
	SRR28341600_2.fastq.gz	Fastqsanger.gz
	SRR28341601_1.fastq.gz	Fastqsanger.gz
	SRR28341601_2.fastq.gz	Fastqsanger.gz
	SRR28341602_1.fastq.gz	Fastqsanger.gz

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Biosynthesis of oxyresveratrol in mulberry (Morus alba L.) is mediated by a group of p-coumaroyl-CoA 2'-hydroxylases acting upstream of stilbene synthases

Project description:Mulberry (Morus alba L.) is considered a millenary medicinal plant and a food source for silkworms. Different M. alba extracts offer a variety of biological and pharmacological properties that are largely attributed to stilbenoids, a small group of phenylpropanoids, including resveratrol and oxyresveratrol. These are naturally present in non-renewable parts of mulberry trees, impeding their efficient extraction. As a way to bypass this spatiotemporal restriction, we generated cell suspensions from mulberry leaf petioles and demonstrated that the combined use of methyl jasmonate and two different types of cyclodextrins were able to elicit a high production of resveratrol and oxyresveratrol. As oxyresveratrol-producing enzymes are still unknown, we improved the structural and functional annotation of the mulberry genome by integrating short and long-read sequencing data and combined it with time series transcriptome, metabolite and proteome data in response to cell elicitation to identify a complete set of phenylpropanoid and stilbenoid related genes. These included 23 stilbene synthase (STS) genes and a group of six p-coumaroyl-CoA 2'-hydroxylases (C2’Hs), all being highly co-expressed with resveratrol and oxyresveratrol production. We transiently transformed grapevine (Vitis vinifera L.) calli and Nicotiana benthamiana plants to functionally validate the role of C2’Hs in the first committed step of oxyresveratrol synthesis, which provides an alternative substrate for STSs by hydroxylating p-coumaroyl-coA into 2’4’-dihydroxycinnamoyl-CoA. Finally, we offer tools for genomic and transcriptomic exploration also in the context of jasmonate elicitation aiding in the characterization of novel stilbenoid-modifying and regulatory genes in the Morus genus.

2025-03-14 | GSE261571 | GEO

Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

Project description:Mulberry (Morus atropurpurea) is an important economic woody tree with rapid growth rate and large biomass, which had great potential for heavy metals remediation. To further understand the mechanisms involved in cadmium accumulation and detoxification in mulberry, we carried out a transcriptomic study to get insights into the molecular mechanisms of the mulberry response to cadmium stress using RNA-seq analysis with BGISEQ-500.

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Effects of Morus alba L. (Mulberry) Leaf Extract in Hypercholesterolemic Mice on Suppression of Cholesterol Synthesis

Project description:To investigate effects of intake of mulberry leaf extracts on hypercholesterolemia, we performed gene expression profiling on rat liver by microarray analysis. Microarray analysis revealed that mulberry leaf extracts up-regulated the gene expression involved in suppression of cholesterol synthesis and stimulation of innate-adaptive Immunity. Mice were fed a high-cholesterol diet without/with orally administration of mulberry leaf extracts for 4 weeks. Livers were taken for RNA extraction and hybridization on Agilent microarrays.

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Gene expression analysis of Ciboria carunculoides-infected Mulberry (Morus atropurpurea) fruit

Project description:Ciboria carunculoides is a major fungal pathogen that infect of mulberry fruit causing popcorn disease leading extensive damage and productivity loss. In spite of such a major impact, mulberry fruit response to C. carunculoides infection is yet to be witnessed. We carried out a transcriptomic study to get insights into the molecular mechanisms and dynamics of the mulberry fruit response to the C. carunculoides infection using RNA-seq analysis with Illumina HiSeq 2000.

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Gene expression analysis of diploid and autotetraploids of Mulberry (Morus atropurpurea)

Project description:Autotetraploid carries several phenotypic changes with larger leaves and fruit compared to diploid. To analysis of phenotypic changes in mulberry autotetraploids on the transcriptome, we performed RNA-Seq analyses on mulberry leaf samples of diploid and autotetraploids using Illumina HiSEq 2000.

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Project description:The mian goal of the study was to identify drought responsive genes of Indian mulberry (Morus alba L.) leaf tissue. Drought stress was imposed at whole plant leve; usig gravimetric approach. Leaf tissue was harvested 14 days post drought stress imposition and used for transcriptome analaysis. Two levels of drought stress (100% and 40% soil field capacity) was maintained by controlled irrigation. The leaf tissue was collected, total RNA was isolated converted to cDNA and used for analysis

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