Project description:In this study, Pinus yunnanensis was used as the experimental material, with a total of 12 samples assigned into four groups: CK_12, CK_6, T5_12 and T9_6. TMT labeling quantitative proteomics technology was applied, and protein detection was performed using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Raw mass spectrometry data were processed for quality control, protein identification and quantification. Differentially expressed proteins (DEPs) among groups were screened. Subsequent bioinformatics analyses were conducted, including GO functional enrichment, KEGG metabolic pathway enrichment, protein-protein interaction network analysis, hub node analysis and protein cluster analysis. This work systematically reveals the alterations in protein expression and underlying molecular regulatory mechanisms of Pinus yunnanensis under different treatments. A total of 319 proteins were identified in this experiment, and multiple sets of significantly differentially expressed proteins were obtained via intergroup comparison. The results provide valuable proteomic data for exploring the physiological and molecular mechanisms of Pinus yunnanensis.
Project description:Plant propagation requires adventitious root production, and the inability to root cuttings prevents some tree species from being widely used and propagated on a big scale. Studies on the differences in adventitious root production ability between species that are easily rooted and those that are difficult to root are few, despite the fact that many studies have been done to examine the developmental mechanisms of adventitious roots. To attempt to address this problem, we examined the transcriptomes of Populus davidiana (difficult-to-root) and Populus yunnanensis (easy-to-root) spikes at various intervals following cutting. We discovered that the number of differential genes was significantly greater in the 96- and 120-hour groups than in the 0- and 48-hour groups. The majority of growth hormone and cytokinin-related genes were up-regulated in P. yunnanensis, while they were down-regulated in P. davidiana.
2026-06-09 | GSE286012 | GEO
Project description:Time-series transcriptome using VISUAL upon cytokinin application
| PRJDB19045 | ENA
Project description:Fungal sequencing of Pinus yunnanensis soil
| PRJNA1161408 | ENA
Project description:Bark tissue Transcriptome of 20 Pinus yunnanensis samples
| PRJNA507489 | ENA
Project description:Transcriptome sequencing of Pinus yunnanensis after nitrogen and phosphorus fertilization