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The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [RNA-Seq]

ABSTRACT: The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [RNA-Seq]

PROVIDER: PRJNA1091356 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28431348.fastq.gz	Fastqsanger.gz
	SRR28431349.fastq.gz	Fastqsanger.gz
	SRR28431350.fastq.gz	Fastqsanger.gz
	SRR28431351.fastq.gz	Fastqsanger.gz
	SRR28431352.fastq.gz	Fastqsanger.gz

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The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [ChIP-seq]

Project description:Grain filling in maize (Zea mays L.) is intricately linked to cell development, involving the regulation of genes responsible for the biosynthesis of storage reserves (starch, proteins, and lipids) and phytohormones. However, the regulatory network coordinating these biological functions remains unclear. In this study, we identified 1,744 high-confidence target genes co-regulated by the transcription factors (TFs) ZmNAC128 and ZmNAC130 (ZmNAC128/130) through chromatin immunoprecipitation sequencing coupled with RNA-seq analysis in the zmnac128/130 loss-of-function mutants. We further constructed a hierarchical regulatory network using DNA affinity purification sequencing analysis of downstream TFs regulated by ZmNAC128/130. In addition to target genes involved in the biosynthesis of starch and zeins, we discovered novel target genes of ZmNAC128/130 involved in the biosynthesis of lipids and indole-3-acetic acid (IAA). Consistently, the number of oil bodies, as well as the contents of triacylglycerol and IAA were significantly reduced in zmnac128/130. The hierarchical regulatory network centered by ZmNAC128/130 revealed a significant overlap between the direct target genes of ZmNAC128/130 and their downstream TFs, particularly in regulating the biosynthesis of storage reserves and IAA. Our results indicated that the biosynthesis of storage reserves and IAA is coordinated by a multi-TFs hierarchical regulatory network in maize endosperm.

2024-06-21 | GSE262335 | GEO

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [RNA-Seq]

2024-06-21 | GSE262336 | GEO

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [DAP-Seq]

2024-06-21 | GSE262337 | GEO

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [ChIP-seq]

Project description:The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [ChIP-seq]

| PRJNA1091354 | ENA

The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [DAP-Seq]

Project description:The biosynthesis of storage reserves and auxin is coordinated by a hierarchical regulatory network in maize endosperm [DAP-Seq]

| PRJNA1091355 | ENA

Auxin regulates endosperm cellularization in Arabidopsis

Project description:The endosperm is an ephemeral tissue that nourishes the developing embryo, similar to the placenta in mammals. In most angiosperms, endosperm development starts as a syncytium, in which nuclear divisions are not followed by cytokinesis. The timing of endosperm cellularization largely varies between species, and the event triggering this transition remains unknown. Here we show that increased auxin biosynthesis in the endosperm prevents its cellularization, leading to seed arrest. Auxin-overproducing seeds phenocopy paternal-excess triploid seeds derived from hybridizations of diploid maternal plants with tetraploid fathers. Concurrently, auxin-related genes are strongly overexpressed in triploid seeds, correlating with increased auxin activity. Reducing auxin biosynthesis and signaling reestablishes endosperm cellularization in triploid seeds and restores their viability, highlighting a causal role of increased auxin in preventing endosperm cellularization. We propose that auxin determines the time of endosperm cellularization, and thereby uncovered a central role of auxin in establishing hybridization barriers in plants.

2019-12-21 | GSE124108 | GEO

Auxin regulates endosperm cellularization in Arabidopsis

Project description:Auxin regulates endosperm cellularization in Arabidopsis

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Parental conflict driven regulation of endosperm cellularization by a family of Auxin Response Factors

Project description:The endosperm is a reproductive tissue supporting embryo development. In most flowering plants, the initial divisions of endosperm nuclei are not succeeded by cellularization; this process occurs only after a specific number of mitotic cycles have taken place. The timing of cellularization significantly influences seed viability and size. Previous research implicated auxin as a key factor in initiating nuclear divisions and determining the timing of cellularization. Here we uncover the involvement of a family of clustered auxin response factors (cARFs) as dosage-sensitive regulators of endosperm cellularization. cARFs, maternally expressed and paternally silenced, are shown to induce cellularization, thereby restricting seed growth. Our findings align with the predictions of the parental conflict theory, suggesting that cARFs represent major molecular targets in this conflict. We further demonstrate a recurring amplification of cARFs in the Brassicaceae, suggesting an evolutionary response to parental conflict by reinforcing maternal control over endosperm cellularization. Our study highlights that antagonistic parental control on endosperm cellularization converges on auxin biosynthesis and signalling.

2024-03-22 | GSE232803 | GEO

Proteome Dynamics of the Cotyledonary Haustorium and Endosperm in the Course of Germination of Euterpe oleracea Seeds

Project description:The role of the cotyledonary haustorium (CH) in the mobilization of nutrient reserves in the endosperm of species of the palm family Arecaceae is a moot question. To shed light on this matter, we present here an analysis of the quantitative proteome changes associated with four developmental stages of CH and three of endosperm during germination. Together, a total of 1965 proteins were identified, being 1538 in the CH and 960 in the endosperm. Both in the CH and endosperm proteomes, we observed an increase in the diversity of hydrolases as the CH and endosperm develops. Qualitative proteomics analysis of four CH developmental stages indicated that each stage is populated by a unique set of proteins and the quantitative analysis data showed an increase in the relative abundance of hydrolases, particularly mannan degrading enzymes, as development progresses. These results add weight to the hypothesis that the CH in the seeds of E. oleracea acts both as a conduit of carbon and nitrogen sources generated by the hydrolysis of the reserves in the endosperm and as a source of hydrolases that will contribute to the mobilization of these reserves.

2021-01-27 | PXD017481 | Pride

Auxin production in the endosperm drives seed coat development in Arabidopsis

Project description:Auxin production in the endosperm drives seed coat development in Arabidopsis

| PRJNA339548 | ENA