Project description:Maternal Asthma During Pregnancy Imprints Fetal Lung Group 2 Innate Lymphoid Cells and Enhances Their Function in Adulthood [scATAC-Seq]
Project description:We investigated the effects on the lungs during fetal in the offspring of asthmatic mothers. Lung ILC2 of offspring of asthmatic mothers altered phenotypically during fetal life.
Project description:Peripheral whole blood transcriptome profiles of pregnant women with normal pregnancy and preeclampsia from 10-18 weeks of gestational age enrolled in the “Vitamin D Antenatal Asthma Reduction Trial” (VDAART).
Project description:We investigated the effects on the lungs during fetal in the offspring of asthmatic mothers. We examined H3K4me3 modification of ILC2 in the adult lungs of offspring of asthmatic mothers.
Project description:In this project, we isolate U1 snRNA associated proteins in Arabidopsis thaliana. We used an antisense oligonucleotide specific for the U1 snRNA and analyzed associated proteins by mass spectrometry. As a control, the same experiments were performed with U2 snRNA- and lacZ-specifc antisense oligonucleotides.
Project description:We investigated the effects on the lungs during fetal in the offspring of asthmatic mothers. ILC2 in the fetal and adult lungs of offspring of asthmatic mothers showed overlapping chromatin accessible altered regions
Project description:We analyzed the total proteome of group 2 innate lymphoid cells (ILC2s) after different stimulation with interleukin-33 (IL-33), a cytokine playing a critical role in human asthma, and TL1A, a TNF-family cytokine also known to activate ILC2s. Upon combined stimulation with IL-33 plus TL1A, we show that lung ILC2s produce high amounts of IL-9 and acquire a transient ‘ILC9’ phenotype. This phenotype is characterized by simultaneous production of large amounts of type 2 cytokines (IL-5, IL-13 and IL-9), induction of the IL-2 receptor CD25 (Il2ra), and of the transcription factors IRF4, JunB and BATF, that form immune-specific complexes known to induce IL-9 expression.