Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA109553 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparative transcriptome profiling of human epidermis keratinocyte stem cells (KSC) versus keratinocyte progenitors (KP)
| PRJNA283140 | ENA
Project description:Development of epidermis includes a complicated program of keratinocyte differentiation. Here we study a new membrane LIM-domain containing Zn-finger protein ZNF185 which is expressed in upper layers of human skin and is up-regulated during keratinocyte differentiation in vitro. Interestingly, depletion of ZNF185 causes delay of keratinocyte differentiation with decreased levels of FLG, LOR, LCEs expression.
2018-08-26 | GSE102613 | GEO
Project description:SAGE libraries from cultured, differentiated keratinocytes and human epidermis, both normal and affected by actinic keratosis Keywords = Keratinocyte, Epidermis, Homo sapiens, Actinic Keratosis, TNF alpha
2002-02-22 | GSE31 | GEO
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Cursons2015 - Regulation of ERK-MAPK
signaling in human epidermis
Model comparing the abundance of
phosphorylated MAPK signalling proteins and calcium signalling in
the epidermis.
This model is described in the article:
Regulation of ERK-MAPK
signaling in human epidermis.
Cursons J, Gao J, Hurley DG, Print
CG, Dunbar PR, Jacobs MD, Crampin EJ.
BMC Syst Biol 2015; 9: 41
Abstract:
The skin is largely comprised of keratinocytes within the
interfollicular epidermis. Over approximately two weeks these
cells differentiate and traverse the thickness of the skin. The
stage of differentiation is therefore reflected in the
positions of cells within the tissue, providing a convenient
axis along which to study the signaling events that occur in
situ during keratinocyte terminal differentiation, over this
extended two-week timescale. The canonical ERK-MAPK signaling
cascade (Raf-1, MEK-1/2 and ERK-1/2) has been implicated in
controlling diverse cellular behaviors, including proliferation
and differentiation. While the molecular interactions involved
in signal transduction through this cascade have been well
characterized in cell culture experiments, our understanding of
how this sequence of events unfolds to determine cell fate
within a homeostatic tissue environment has not been fully
characterized.We measured the abundance of total and
phosphorylated ERK-MAPK signaling proteins within
interfollicular keratinocytes in transverse cross-sections of
human epidermis using immunofluorescence microscopy. To
investigate these data we developed a mathematical model of the
signaling cascade using a normalized-Hill differential equation
formalism.These data show coordinated variation in the
abundance of phosphorylated ERK-MAPK components across the
epidermis. Statistical analysis of these data shows that
associations between phosphorylated ERK-MAPK components which
correspond to canonical molecular interactions are dependent
upon spatial position within the epidermis. The model
demonstrates that the spatial profile of activation for
ERK-MAPK signaling components across the epidermis may be
maintained in a cell-autonomous fashion by an underlying
spatial gradient in calcium signaling.Our data demonstrate an
extended phospho-protein profile of ERK-MAPK signaling cascade
components across the epidermis in situ, and statistical
associations in these data indicate canonical ERK-MAPK
interactions underlie this spatial profile of ERK-MAPK
activation. Using mathematical modelling we have demonstrated
that spatially varying calcium signaling components across the
epidermis may be sufficient to maintain the spatial profile of
ERK-MAPK signaling cascade components in a cell-autonomous
manner. These findings may have significant implications for
the wide range of cancer drugs which therapeutically target
ERK-MAPK signaling components.
This model is hosted on
BioModels Database
and identified by:
BIOMD0000000659.
To cite BioModels Database, please use:
Chelliah V et al. BioModels: ten-year
anniversary. Nucl. Acids Res. 2015, 43(Database
issue):D542-8.
To the extent possible under law, all copyright and related or
neighbouring rights to this encoded model have been dedicated to
the public domain worldwide. Please refer to
CC0
Public Domain Dedication for more information.
2024-09-02 | BIOMD0000000659 | BioModels
Project description:SAGE libraries from cultured, differentiated keratinocytes and human epidermis, both normal and affected by actinic keratosis Keywords = Keratinocyte, Epidermis, Homo sapiens, Actinic Keratosis, TNF alpha
2002-02-21 | E-GEOD-31 | biostudies-arrayexpress
Project description:Keratinocyte detachment-differentiation connection
| PRJNA245312 | ENA
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
2011-02-19 | GSE20463 | GEO
Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.
| S-EPMC2531291 | biostudies-literature
Project description:Cutaneous squamous cell carcinoma (cSCC) is the second most common human tumor and arises from keratinocytes that comprise the epidermis and its associated hair follicles. Remarkably, transformed keratinocytes within a cSCC retain some ability to execute the terminal differentiation program that typically generates a stratified epidermis. Instead of the polarized stratification towards the body surface seen in normal epidermis, cSCC keratinocytes differentiate concentrically inward to produce keratin pearls. It is well appreciated that cSCC differentiation status is correlated with degree of local invasion and potential for distant metastasis, with well differentiated tumors having a better prognosis. While molecular mechanisms that govern the differentiation process are incompletely understood, identifying candidate molecules associated with cSCC differentiation can provide new histologic markers for prognostic stratification. Here we demonstrate that Flower (FWE), a newly described regulator of lamellar body (LB) trafficking in normal epidermis, is specifically expressed within highly differentiated layers of cSCC. Genetic knockout of the hFWE gene dysregulates cornification and LB-related gene expression leading to abnormal keratinization patterns, while ectopic hFWE4 expression drives G1-arrest and exit from the proliferative basal keratinocyte population. Additionally, we find that poorly differentiated keratinocyte regions of human cSCC exhibit minimal FWE-positivity, while this population is readily detectable in well-differentiated regions. We propose that FWE represents a novel differentiation marker in cSCC that could be utilized for classifying differentiation status and prognostic stratifying these tumors.
2026-02-24 | GSE314399 | GEO