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ABSTRACT: The developmental proteome and transcriptome of Bombyx mori

PROVIDER: PRJNA1096142 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28553000.fastq.gz	Fastqsanger.gz
	SRR28553001.fastq.gz	Fastqsanger.gz
	SRR28553002.fastq.gz	Fastqsanger.gz
	SRR28553003.fastq.gz	Fastqsanger.gz
	SRR28553004.fastq.gz	Fastqsanger.gz

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Project description:We here provide a comprehensive paired proteome and transcriptome data set including major stages (egg, larva, pupa and adult) across the whole developmental life cycle of the silkworm Bombyx mori. Our data constitutes a rich resource enabling comparative analysis of developmental regulatory dynamics. Analyzing this paired proteome and transcriptome data we revealed similarities and differences between both levels of gene expression in B. mori. We were also able to examine protein-transcript correlations and characterize stage-specific dynamics. Integrating similar publicly available data for D. melanogaster were also compared the two holometabolous insects at the level of the developmental proteome and transcriptome.

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SILKWORM WING SCALES LC-MSMS ANALYSIS RAW DATA

Project description:To identify the cuticular proteins in developing wing scales of Bombyx mori, we performed LC-MS/MS analysis of dissoliving developing wing scales from Bombyx mori

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Project description:Molecular genetic studies of Bombyx mori have led to profound advances in understanding the regulation of development. Bombyx mori brain, as a main endocrine organ, plays important regulatory roles in various biological processes. The microarray technology will allow the genome-wide analysis of gene expression patterns in silkworm brains. We reported microarray-based gene expression profiles in silkworm brains at four stages including V7, P1, P2 and P3. A total of 4,550 genes were transcribed in at least one selected stage. Of these, clustering algorithms separated the expressed genes into stable expressed genes and variable expressed genes. The results of the gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analysis of stable expressed genes showed that the ribosomal and oxidative phosphorylation pathways were principal pathways. Secondly, four clusters of genes with significantly different expression patterns were observed in the 1,175 variable expressed genes. Thirdly, thirty-two neuropeptide hormones genes, nine neuropeptide-like precursor genes, and 117 cuticular protein genes were expressed in selected developmental stages. The present study deﬁned major characteristics of the transcriptional profiles in the brains of Bombyx mori at the specific development stages. Our data will provide abundant information that will be useful in future research. Transcription profiling experiments, 4 developmental stages (samples) were analyzed. Dual-channel experiments, with test samples labeled by Cy5 and common reference samples labeled by Cy3. Common reference sample was used for data normalization. One Biological replicate. No dye-swaps.

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