Project description:The Agilent SurePrint G3 Mouse GE V2.0 Microarray was used in this experiment to analyze data of the 6 samples. Goal was to determine the differential genes of Sham and Bilateral carotid artery stenosis (BCAS).
Project description:Experimental atherosclerosis was established by feeding Apoe KO mice a western diet(WD) for 14 weeks and experimental cerebral ischemia was established by bilateral carotid artery stenosis (BCAS). RNA-seq was perfomed to investigate the potential gene regulation of AS-Exos-dependent changes of microglia in the corpus callosum after BCAS.
Project description:This SuperSeries is composed of the following subset Series: GSE20680: Whole Blood Cell Gene Expression Profiling in Patients with Coronary Artery Disease from the Cathgen Registry GSE20681: Whole Blood Cell Gene Expression Profiling in Patients with Coronary Artery Disease from the PREDICT Trial Refer to individual Series
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Carotid artery stenosis (CAS) is a major cause of cardiovascular diseases, such as myocardial infarction and stroke. Mitochondrial dysfunction can trigger the onset of these conditions. CUB Domain Containing Protein 1 (CDCP1) is a transmembrane protein that has been demonstrated to contribute to various tumors and myocardial infarction; however, its role in carotid artery stenosis has not yet been reported. In this study, we investigated how CDCP1 affects cellular activity by regulating mitochondrial autophagy, and we propose that 8-isopentenylnaringenin (8-PN) may promote autophagy and alleviate carotid artery stenosis by reducing the expression of CDCP1. CDCP1 is upregulated in vascular smooth muscle cells within a carotid artery stenosis model, as demonstrated by studies conducted both in vivo and in vitro. Knocking down CDCP1 enhanced mitochondrial autophagy by upregulating ATG4A, leading to a reduction in reactive oxygen species (ROS) release and protection of cell viability. Moreover, both in vitro and in vivo experiments confirmed that 8-PN effectively decreases CDCP1 expression, activates cellular autophagy, and mitigates the progression of carotid artery stenosis. These findings highlight the importance ofCDCP1 in carotid artery stenosis and underscore the capability as a therapeutic option for this condition.
