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ABSTRACT: Haloxylon ammodendron rhizosphere soil 16S ribosomal RNA

PROVIDER: PRJNA1098027 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR28615177_1.fastq.gz	Fastqsanger.gz
	SRR28615177_2.fastq.gz	Fastqsanger.gz
	SRR28615178_1.fastq.gz	Fastqsanger.gz
	SRR28615178_2.fastq.gz	Fastqsanger.gz
	SRR28615179_1.fastq.gz	Fastqsanger.gz

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De novo transcription analysis of Haloxylon ammodendron in response to osmotic stress

Project description:Purpose: To elucidate the potential molecular mechanisms that are involved in drought tolerance of Haloxylon ammodendron. Method: Pathways and candidate genes drought-resistant genes were identified in shoots and roost of Haloxylon ammodendron under sorbitol treatment for 6 and 24h using RNA-seq and Digital Gene Expression. Results: 3,353 differently expressed genes in shoots and 4,564 in roots were successfully indentified. Conclusion: A detailed investigation of the pathways and candidate genes identified in this study promote the research on the molecular mechanisms of drought resistance in the xerophytic species, and lay a solid foundation for developing stress-tolerant forage and crop species by using excellent genes relevant to drought tolerance in Haloxylon. ammodendron.

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Project description:Understanding the environmental factors that shape microbial communities is crucial, especially in extreme environments, like Antarctica. Two main forces were reported to influence Antarctic soil microbes: birds and plants. Both birds and plants are currently undergoing unprecedented changes in their distribution and abundance due to global warming. However, we need to clearly understand the relationship between plants, birds and soil microorganisms. We therefore collected rhizosphere and bulk soils from six different sampling sites subjected to different levels of bird influence and colonized by Colobanthus quitensis and Deschampsia antarctica in the Admiralty Bay, King George Island, Maritime Antarctic. Microarray and qPCR assays targeting 16S rRNA genes of specific taxa were used to assess microbial community structure, composition and abundance and analyzed with a range of soil physico-chemical parameters. The results indicated significant rhizosphere effects in four out of the six sites, including areas with different levels of bird influence. Acidobacteria were significantly more abundant in soils with little bird influence (low nitrogen) and in bulk soil. In contrast, Actinobacteria were significantly more abundant in the rhizosphere of both plant species. At two of the sampling sites under strong bird influence (penguin colonies), Firmicutes were significantly more abundant in D. antarctica rhizosphere but not in C. quitensis rhizosphere. The Firmicutes were also positively and significantly correlated to the nitrogen concentrations in the soil. We conclude that the microbial communities in Antarctic soils are driven both by bird and plants, and that the effect is taxa-specific.

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