Project description:Tomato plants are submitted to a high diversity of herbivory pests, among them the leafminer Tuta absoluta, considered as one of the most important threat on the tomato worldwide production. In spite of its susceptibility to this pest, a better understanding of the tomato plant response to T. absoluta herbivory will help defining plant resistance traits and enlarging the range of possibilities for an efficient integrated pest management strategy. We analyzed the transcriptomic response in leaves of tomato (cv. Better Bush) submitted to the herbivory of T. absoluta larvae after 5h and 24h.
2022-04-18 | GSE200795 | GEO
Project description:Research on Integrated approaches for pest and disease management in horticultural crops
Project description:Fungal entomopathogens like Beauveria bassiana (Bals.) Vuill. (Ascomycota: Hypocreales) are known as antagonist of insects with multiple functional and ecological roles and have attracted increased attention as biocontrol agents in integrated pest management programs. A microarray analysis was performed to work out fundamental aspects of genes involved in the interaction between grapevine and the endophytic fungus B. bassiana. The results indicate an up-regulation of diverse defense-related genes in grapevine as a response to a treatment with B. bassiana
Project description:Insect sex pheromones are an important resource in integrated pest management. Encouraging steps have been made towards their sustainable production in plant biofactories, but important obstacles remain to be resolved. We have used transcriptomic analysis to determine the molecular perturbations behing growth penalty associated with high pheromone production in transgenic Nicotiana benthamiana lines with constitutive expression of genes for biosynthesis of moth sex pheromones - SexyPlants v1.2. We analyzed two lines with high production of pheromones as well as wt N. benthamiana.
Project description:Insect sex pheromones are an important resource in integrated pest management. Encouraging steps have been made towards their sustainable production in plant biofactories, but important obstacles remain to be resolved. We have used transcriptomic analysis to determine the molecular perturbations behing growth penalty associated with high pheromone production in transgenic Nicotiana benthamiana lines with constitutive expression of genes for biosynthesis of moth sex pheromones - SexyPlants v1.0. We analyzed lines with high production (homozygous lines) and strong growth penalty as well as lines with low production (heterozygous lines) and negligible growth penalty.
Project description:Seed defence priming is emerging as a novel, cost-efficient and environmentally safe tool for pest management. Previous reports suggest that this strategy could potentially uncouple the defence-growth trade-off in plants, but this role and the mechanisms underlying it remain elusive. Here, we investigated the ability of seed priming with four phytohormones and two plant vitamins to uncouple the growth-defence trade-off in Arabidopsis thaliana upon pest infestation, focusing on its molecular dynamics. To broaden our scope, two phytophagous species from different feeding guilds were used: the generalist cell-content sucker Tetranychus urticae, and the specialist chewer Pieris brassicae. A combined -omics approach revealed distinct and interactive effects of seed priming and herbivory. Specifically, seed priming with 0.1 mM methyl jasmonate induced a state of readiness characterized by reduced stomatal aperture, high transcriptomic variation, increased sugar content and enhanced translational rate. Upon pest infestation, primed plants exhibited herbivore-specific responses. Interaction of seed priming and T. urticae infestation increased amino acid levels, hydroxycinnamic acid amide production, and reallocated resources from arabidopsides and anthocyanin biosynthetic pathways. In contrast, P. brassicae infestation in primed plants led to enhanced anthocyanin biosynthesis, alterations in lignin metabolic intermediates, and sustained carbon and nitrogen sources. These findings demonstrate that seed priming can enhance pest-specific defences in plants without incurring a fitness penalty, effectively managing infestations while maintaining growth.
Project description:The leaf miner, Tuta absoluta, is a notorious destructive pest of tomato that causes important losses worldwide. Breeding of resistant/tolerant tomato cultivars could be an effective strategy for T. absoluta management but, despite the economic importance of tomato, very limited information is available about its response to this treat. To elucidate the defense mechanisms to the herbivore feeding a comparative analysis was performed between a tolerant and susceptible cultivated tomato at both morphological and at transcriptome level to highlight constitutive leaf barriers, molecular and biochemical mechanisms to counter the effect of T. absoluta attack. The tolerant genotype showed an enhanced constitutive barrier due to the higher density of trichomes and increased inducible reactions upon mild infestation thanks to the activation/repression of key transcription factors regulating a higher number of genes involved in cuticle formation and cell wall strength as well as of antinutritive enzymes, and genes involved in the production of chemical toxins and bioactive secondary metabolites. Overall, our findings provide an important foundation for further understanding the insect resistance mechanisms of resistant/tolerant varieties that can be exploited for developing T. absoluta tolerant cultivars, acting as important component of integrated pest management strategy for more sustainable production.
Project description:The entomopathogenici fungus B. bassiana is of great imporatnce in biological control of insect pests. In integrated pest management (IPM) program, unsaturated fatty acids and their derivatives are used as fungicides. The compatibility of mycoinsecticides with fungicides has attracted more and more attention. The genome-wide exprssion analysis involved in fungal resonse to unsaturated fatty acids was analyzed by using high throughput sequencing (RNA-Seq) at different concentrations of linoleic acid. Our transcriptional profiles revealed that numerous differentially expressed genes (DEGs), of which involved in metabolism, cell transport and cell rescue, were significantly involved in the tolerance of B. bassiana to linoleic acid stress.