Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: WGS Mutants of the SREBP pathway in Xanthophyllomyces dendrorhous

PROVIDER: PRJNA1098865 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28621941_1.fastq.gz	Fastqsanger.gz
	SRR28621941_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Sterol Regulatory Element-Binding Protein Sre1 regulates carotenogenesis in the red yeast Xanthophyllomyces dendrorhous.

Project description:Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces carotenoids, mainly astaxanthin. Astaxanthin is an organic pigment of commercial interest due to its antioxidant and coloring properties. X. dendrorhous has a functional Sterol Regulatory Element-Binding Protein (SREBP) pathway, and the Sre1 protein is the SREBP homolog in this yeast. However, how Sre1 is promoting the biosynthesis of sterols and carotenoids in X. dendrorhous is unknown. In this work, comparative RNA-seq analysis between modified X. dendrorhous strains that have an active Sre1 protein and the wild-type was performed to identify Sre1-dependent genes. In addition, Sre1 direct target genes were identified through chromatin immunoprecipitation combined with lambda exonuclease digestion (ChIP-exo) assays. SRE motifs were detected in the promoter regions of several Sre1 direct target genes and were consistent with the SREs described in other yeast species. Sre1 directly regulates genes related to ergosterol biosynthesis as well as genes related to the mevalonate pathway, which synthesizes the building blocks of isoprenoids, including carotenoids. Two carotenogenic genes, crtE and crtR, were also identified as Sre1 direct target genes. Thus, carotenogenesis in X. dendrorhous is regulated by Sre1 through the regulation of the mevalonate pathway and the regulation of the crtE and crtR genes. As the crtR gene encodes a cytochrome P450 reductase, Sre1 regulates pathways that include cytochrome P450 enzymes, such as the biosynthesis of carotenoids and sterols. These results demonstrate that Sre1 is a sterol master regulator that is conserved in X. dendrorhous.

2020-09-29 | GSE152739 | GEO

Xanthophyllomyces dendrorhous whole genome and transcriptome analyses

Project description:Xanthophyllomyces dendrorhous

| PRJEB6925 | ENA

Phaffia rhodozyma

Project description:Xanthophyllomyces dendrorhous overview

| PRJNA283429 | ENA

Phaffia rhodozyma strain:CBS 6938

Project description:Xanthophyllomyces dendrorhous - Genome sequencing and assembly

| PRJNA637109 | ENA

Phaffia rhodozyma

Project description:NBRP: Genome sequencing of Xanthophyllomyces dendrorhous JCM 9681

| PRJDB3716 | ENA

Project description:Xanthophyllomyces dendrorhous CBS 6938 mutant strain gene expression profile

| PRJNA517352 | ENA

Phaffia rhodozyma strain:UBV-AX4

Project description:Xanthophyllomyces dendrorhous UBV-AX4 Gene Expression Profiling - XDOP_H2O2-1 transcriptome

| PRJNA650601 | ENA

Phaffia rhodozyma strain:UBV-AX4

Project description:Xanthophyllomyces dendrorhous UBV-AX4 Gene Expression Profiling - XDOP_H2O2-3 transcriptome

| PRJNA650603 | ENA

Phaffia rhodozyma strain:UBV-AX4

Project description:Xanthophyllomyces dendrorhous UBV-AX4 Gene Expression Profiling - XDOP_dark-1 transcriptome

| PRJNA650604 | ENA

Phaffia rhodozyma strain:UBV-AX4

Project description:Xanthophyllomyces dendrorhous UBV-AX4 Gene Expression Profiling - XDOP_dark-2 transcriptome

| PRJNA650605 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data