Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1099158 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Insect stress modulates phenylpropanoid and phytohormone signaling to impair Sorghum defense responses in COMT bmr12 mutant at 10dpi
| PRJNA1120573 | ENA
Project description:Schengen-pathway modulates the phenylpropanoid pathway and induces defense-related mechanisms
2020-11-01 | GSE158809 | GEO
Project description:Systemin was identified in 1991 as the first plant signaling peptide, and is required for defense against insect herbivores and necrotrophic pathogens in tomato plants. Systemin was first conceived as a hormone-like, long-distance messenger mediating the activation of systemic defense responses far from the site of insect attack. It was later shown to rather act as a phytocytokine, amplifying the local wound response for production of downstream signals resulting in defense gene activation in distal tissues. Systemin perception and signaling rely on the systemin receptor SYR1. However, SYR1-dependent signaling, and how systemin signaling differs from other peptide-mediated signaling pathways, is largely unknown. Here we used a Solanum peruvianum suspension cell culture to analyze phospho-proteomic responses triggered by 10 nM systemin. Samples were analyzed in a time series of 0, 1, 2, 5, 15, and 45 min after systemin treatment. To identify systemin-specific events a mutant cell culture lacking the SYR1 systemin receptor was analyzed in parallel. The experiment was performed in six biological replicates. Samples numbering scheme: Genotype_TreatmentTimeReplicate(e.g.,WT_S53 corresponding to wild-type cells treated with systemin, for 5min, the 3rd biological replicates)
2025-06-17 | PXD054229 | Pride
Project description:Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of many plant species including Arabidopsis thaliana. The symbiotic interaction promotes plant per-formance, growth and resistance/tolerance against abiotic and biotic stress. We demonstrate that exudated compounds from the fungus activate stress and defense responses in the Arabidopsis roots and shoots before the two partners are in physical contact. They induce stomata closure, stimulate reactive oxygen species (ROS) production, stress-related phytohormone accumulation and activate defense and stress genes in the roots and/or shoots. Once a physical contact is established, the stomata re-open, ROS and phytohormone levels decline, and the gene expression pattern indicates a shift from defense to mutualistic interaction. We propose that exudated compounds from P. indica induce stress and defense responses in the host. Root colonization results in the downregulation of defense responses and the activation of genes involved in promoting plant growth, metabolism and performance.
2016-05-30 | GSE58771 | GEO
Project description:Arabidopsis thaliana plants fend off insect attack by constitutive and inducible production of toxic metabolites such as glucosinolates (GS). A triple mutant lacking MYC2, MYC3, and MYC4, three basic helix-loop-helix transcription factors that are known to additively control jasmonate-related defense responses, is highly susceptible to insect herbivory. In this study, we performed a whole-genome microarray analysis on three-week-old plants and compared wild-type (Col-0) and myc2myc3myc4 plants in control conditions (no treatment). Myc234 was shown to have a highly reduced expression of GS biosynthesis genes.
2013-08-27 | E-MTAB-1777 | biostudies-arrayexpress
Project description:MYC2 is a central node controlling the crosstalk between JA and other phytohormone signalling pathways and regulating responses to light and circadian clock. We hypothesized that this transcription factor might coordinate prioritization of microbiota-induced growth over defense under suboptimal light conditions
2020-10-27 | GSE160115 | GEO
Project description:Next-generation sequencing (NGS)-derived transcriptomic profiling was carried out to compare the transcriptomic changes mediated by COMT knockout in U87 cells. We found significant enrichment of gene signatures involving IFNa/b signaling in COMT-KO U87 cells compared to control cells. Our analysis also demonstrated that COMT deficiency interferes with mitochondrial functions and induces the expression of genes associated with the anti-viral RNA sensing pathway.
2023-10-12 | GSE237053 | GEO
Project description:<p>Plants exhibit dynamic physiological and biochemical responses to herbivory that often involve extensive metabolic reprogramming. Withania somnifera (Ashwagandha), a globally important medicinal plant, is susceptible to herbivory by the pest Henosepilachna vigintioctopunctata, resulting in significant yield losses. Understanding herbivory-induced metabolic responses is essential for elucidating plant-insect interactions and identifying potential defense-associated metabolites. In this study, we investigated the metabolic responses of W. somnifera leaves to H. vigintioctopunctata infestation using comprehensive metabolomics approach combining liquid chromatography-high-resolution accurate mass spectrometry (LC-HRAMS) and gas chromatography-mass spectrometry (GC-MS). Our findings revealed that herbivore triggers a significant metabolic shift by upregulating secondary metabolites, withanosides and phenylpropanoids. More importantly, hormones such as IAA and salicylic acid are involved in the signaling of defense mechanisms in W. somnifera. Furthermore, primary metabolites from carbohydrate metabolism were significantly affected. In addition, elemental analysis using ICP-OES revealed the accumulation of micronutrients due to pest infestation in W. somnifera. Notably, we also found plant defense metabolites specifically withanolides and withanosides within the herbivore pests themselves. The accumulation of withanosides in response to pest infestation was further confirmed through gene expression analysis. Additionally, feeding on Withania leaf extract containing withanolides adversely affected beetle physiology and survival. Collectively, our findings demonstrate that herbivory induces substantial metabolic plasticity in W. somnifera, particularly involving withanolide glycosylation and phenylpropanoid metabolism. This study provides a valuable framework for understanding herbivory-associated metabolic responses and identifies candidate pathways for future functional and comparative studies.</p>
2026-02-17 | MTBLS13904 | MetaboLights
Project description:Piriformospora indica, an endophytic fungus of Sebacinales, colonizes the roots of many plant species including Arabidopsis thaliana. The symbiotic interaction promotes plant per-formance, growth and resistance/tolerance against abiotic and biotic stress. We demonstrate that exudated compounds from the fungus activate stress and defense responses in the Arabidopsis roots and shoots before the two partners are in physical contact. They induce stomata closure, stimulate reactive oxygen species (ROS) production, stress-related phytohormone accumulation and activate defense and stress genes in the roots and/or shoots. Once a physical contact is established, the stomata re-open, ROS and phytohormone levels decline, and the gene expression pattern indicates a shift from defense to mutualistic interaction. We propose that exudated compounds from P. indica induce stress and defense responses in the host. Root colonization results in the downregulation of defense responses and the activation of genes involved in promoting plant growth, metabolism and performance. Twelve day-old (48 h cold treatment and 10 days of illumination) Arabidopsis seedlings of equal sizes were selected for co-cultivation experiments. They were transferred to PNM plates with a nylone membrane on the top (Johnson et al. 2011) and exposed to a fungal plug 5 mm in diameter or a KM plug of the same size without fungal hyphae (control). The plugs were placed 3 cm away from the closest root part . The light intensity (80 ± 5 μmol m-2 sec-1) was checked every third day to ensure that both P. indica- and mock-treated seedlings receive equal amounts of light.
2016-05-30 | E-GEOD-58771 | biostudies-arrayexpress