Project description:The leaf of Eriobotrya japonica (Ej) has been used for a long time as an oriental medicine to treat pulmonary inflammatory diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the leaves of Ej when challenged with LPS using a microarray chip.
Project description:The leaf of Eriobotrya japonica (Ej) has been used for a long time as an oriental medicine to treat pulmonary inflammatory diseases. This study investigated the gene expression changes by lipopolysaccharide (LPS) stimulation in the cultured human gingival fibroblast and the gene expression changes by the leaves of Ej when challenged with LPS using a microarray chip. Human gingival fibroblast were divided into three experimental groups; â C: Control, â¡ LPS: LPS-treatment only, ⢠LPS-Ej: LPS- and Ej-treatments. Total RNA was isolated from each experimental fibroblast (3 experimental group à 1 sample of each experimental group = total 3 samples).
