Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [scRNA-seq]

ABSTRACT: IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [scRNA-seq]

PROVIDER: PRJNA1100580 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28701662.fastq.gz	Fastqsanger.gz
	SRR28701663.fastq.gz	Fastqsanger.gz
	SRR28701664.fastq.gz	Fastqsanger.gz
	SRR28701665.fastq.gz	Fastqsanger.gz
	SRR28701666.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [ChIP-seq]

Project description:Little is known about how interferon regulatory factor 7 (IRF7) promotes systemic autoimmunity. Here performed scRNAseq on B cells purified from the spleens of 3 month old FcgRIIB-/-IRF7-/- and FcgRIIB-/- controls. We identified a role for IRF7 in promoting the differentiation of B cells through the GC and PC pathways during an autoimmune response. To determine which genes IRF7 specifically controlled for this response, we purified class-switched activated B cells from FcgRIIB-/- mice and performed IRF7 ChIPseq. We identified that IRF7 transcirptionally regulated genes assocaited with B cell protein translation and metabolism. In summary, we have identified a B cell intrinsic role for IRF7 in driving the transcriptoinal profile required for GC and PC differentiation in SLE-like autoimmunity.

2025-03-01 | GSE264034 | GEO

IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [scRNA-seq]

2025-03-01 | GSE264033 | GEO

IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [ChIP-seq]

Project description:IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [ChIP-seq]

| PRJNA1100581 | ENA

Proteome profiling of mouse germinal center B cells

Project description:Productive B cell responses are critical to protect a host from infection. The spleen and lymph nodes are populated by resting follicular B cells, which can enter germinal centers upon antigen encounter. Once in the germinal center, B cells migrate between the dark and light zones, where they undergo somatic hypermutation and selection, respectively. While germinal center B cells have been studied, an intense molecular understanding of these cells/subsets (and the differences between them) is lacking.

2020-04-06 | PXD015524 | Pride

ChIPseq analyses in germinal center B cells

Project description:Germinal center B cells were isolated from human tonsil tissue and crosslinked. ChIP was performed on two distinct pools of germinal center cells, each obtained from 3-5 donors. The experiment includes two biological replicates (germinal center cell pools from different donors). ChIP was performed on both pools and subject to library preparation and sequencing. Input DNA was sequenced for both pools.

2015-12-04 | E-GEOD-68349 | biostudies-arrayexpress

Data regarding transplant induced germinal center humoral autoimmunity.

Project description:This data is related to the research article entitled "Germinal center humoral autoimmunity independently mediates progression of allograft vasculopathy" (Harper et al., 2016) [2]. The data presented here focuses on the humoral autoimmune response triggered by transferred allogeneic CD4 T cells and includes details on: (a) the recipient splenic germinal center (GC) response; (b) augmentation of humoral autoimmunity and accelerated heart allograft rejection following transplantation from donors primed against recipient; (c) flow cytometric analysis of donor and recipient CD4 T cells for signature markers of T follicular helper cell differentiation; (d) in vitro donor endothelial cell migration in response to column purified autoantibody from recipient sera; (e) analysis of development of humoral responses in recipients following adoptive transfer of donor CD4 T cells and; (f) the development of humoral autoimmunity in mixed haematopoietic chimeric mice.

| S-EPMC6327748 | biostudies-literature

Deletion of miR-15a/16-1 in murine germinal center B-cells.

Project description:Given the tumor suppressing function of miR-15a/16-1 cluster, we studied its role in the germinal center B-cells that give rise to most lymphoid malignancies.

2021-09-09 | PXD021323 | Pride

B cell-derived IL-6 initiates spontaneous germinal center formation during systemic autoimmunity.

Project description:Recent studies have identified critical roles for B cells in triggering autoimmune germinal centers (GCs) in systemic lupus erythematosus (SLE) and other disorders. The mechanisms whereby B cells facilitate loss of T cell tolerance, however, remain incompletely defined. Activated B cells produce interleukin 6 (IL-6), a proinflammatory cytokine that promotes T follicular helper (TFH) cell differentiation. Although B cell IL-6 production correlates with disease severity in humoral autoimmunity, whether B cell-derived IL-6 is required to trigger autoimmune GCs has not, to our knowledge, been addressed. Here, we report the unexpected finding that a lack of B cell-derived IL-6 abrogates spontaneous GC formation in mouse SLE, resulting in loss of class-switched autoantibodies and protection from systemic autoimmunity. Mechanistically, B cell IL-6 production was enhanced by IFN-γ, consistent with the critical roles for B cell-intrinsic IFN-γ receptor signals in driving autoimmune GC formation. Together, these findings identify a key mechanism whereby B cells drive autoimmunity via local IL-6 production required for TFH differentiation and autoimmune GC formation.

| S-EPMC5679179 | biostudies-literature

CRTC2 couples genotoxic stress and plasma cell differentiation in the germinal center

Project description:This SuperSeries is composed of the following subset Series: GSE23169: Transcriptional profiling of Ramos germinal center B cells GSE23170: ChIP-on-chip experiment from Ramos cells to analyze genome-wide CRTC2 binding sites in germinal center B cells Refer to individual Series

2012-01-01 | E-GEOD-23171 | biostudies-arrayexpress

ChIP-seq for IRF7

Project description:We performed ChiP-Seq for IRF7 in GM-CSF induced bone-marrow cells. IRF7 ChIP-Seq in myeloid cells

2014-12-03 | E-GEOD-62697 | biostudies-arrayexpress