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IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [ChIP-seq]

ABSTRACT: Little is known about how interferon regulatory factor 7 (IRF7) promotes systemic autoimmunity. Here performed scRNAseq on B cells purified from the spleens of 3 month old FcgRIIB-/-IRF7-/- and FcgRIIB-/- controls. We identified a role for IRF7 in promoting the differentiation of B cells through the GC and PC pathways during an autoimmune response. To determine which genes IRF7 specifically controlled for this response, we purified class-switched activated B cells from FcgRIIB-/- mice and performed IRF7 ChIPseq. We identified that IRF7 transcirptionally regulated genes assocaited with B cell protein translation and metabolism. In summary, we have identified a B cell intrinsic role for IRF7 in driving the transcriptoinal profile required for GC and PC differentiation in SLE-like autoimmunity.

ORGANISM(S): Mus musculus

PROVIDER: GSE264034 | GEO | 2025/03/01

REPOSITORIES: GEO

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IRF7 promotes systemic autoimmunity by transcriptionally regulating B cell differentiation through germinal center and plasma cell pathways [scRNA-seq]

Project description:Little is known about how interferon regulatory factor 7 (IRF7) promotes systemic autoimmunity. Here performed scRNAseq on B cells purified from the spleens of 3 month old FcgRIIB-/-IRF7-/- and FcgRIIB-/- controls. We identified a role for IRF7 in promoting the differentiation of B cells through the GC and PC pathways during an autoimmune response. To determine which genes IRF7 specifically controlled for this response, we purified class-switched activated B cells from FcgRIIB-/- mice and performed IRF7 ChIPseq. We identified that IRF7 transcirptionally regulated genes assocaited with B cell protein translation and metabolism. In summary, we have identified a B cell intrinsic role for IRF7 in driving the transcriptoinal profile required for GC and PC differentiation in SLE-like autoimmunity.

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