Project description:Coelastrella tenuitheca strain S93-3 is a green microalga that demonstrates remarkable tolerance to heavy metal stress. It is a promising candidate for bioremediation applications, strongly resistant to cadmium, and an essential organism for phytoremediation studies. However, the molecular mechanism of the response of C. tenuitheca S93-3 to cadmium stress, and the key regulatory genes for specific detoxification processes are poorly understood.
Project description:Purpose: The goal of this study is to compare endothelial small RNA transcriptome to identify the target of OASL under basal or stimulated conditions by utilizing miRNA-seq. Methods: Endothelial miRNA profilies of siCTL or siOASL transfected HUVECs were generated by illumina sequencing method, in duplicate. After sequencing, the raw sequence reads are filtered based on quality. The adapter sequences are also trimmed off the raw sequence reads. rRNA removed reads are sequentially aligned to reference genome (GRCh38) and miRNA prediction is performed by miRDeep2. Results: We identified known miRNA in species (miRDeep2) in the HUVECs transfected with siCTL or siOASL. The expression profile of mature miRNA is used to analyze differentially expressed miRNA(DE miRNA). Conclusions: Our study represents the first analysis of endothelial miRNA profiles affected by OASL knockdown with biologic replicates.
