Project description:Coelastrella tenuitheca strain S93-3 is a green microalga that demonstrates remarkable tolerance to heavy metal stress. It is a promising candidate for bioremediation applications, strongly resistant to cadmium, and an essential organism for phytoremediation studies. However, the molecular mechanism of the response of C. tenuitheca S93-3 to cadmium stress, and the key regulatory genes for specific detoxification processes are poorly understood.
Project description:Puccinia graminis f. sp. tritici is the cause of wheat stem rust. A microarray was designed from genes predicted from the P. graminis f. sp. tritici genome assembly, and gene expression measured for four conditions which include wheat or barley infecting growth stages initiated by urediniospores. mRNA was prepared from fresh urediniospores, uredinospores germinated for 24 hr, wheat seedlings infected with urediniospores for 8 days, and barley seedlings infected with urediniospores for 8 days. The asexual uredinial infection cycle on wheat produces additional urediniospores, which can start new cycles of wheat infection and are readily spread by aerial transport. This expression data is further described in Duplessis et al, Obligate Biotrophy Features Unraveled by the Genomic Analysis of the Rust Fungi, Melampsora larici-populina and Puccinia graminis f. sp. tritici
