Project description:The whole regulon of the LTTR All3953 was determined at 3 h after Ci deficiency in the cyanobacterium Anabaena sp. PCC 7120 by ChIP-Seq analysis. A TAP-tagged version of the protein was used for the chromatin immunoprecipitation. A total of 142 peaks were found, mainly located in the chromosome of Anabaena.
Project description:In order to examine the response of Anabaena sp. PCC 7120 to an infection of the Cyanophage A1, we have used customized microarrays to identify genes potentially up- or downregulated by the infection. For this we compared cell culture before and after infection.
Project description:To examine Alr3614 influence on the expression of Anabaena sp. PCC 7120, we have used customized microarrays to identify genes potentially regulated by this short DNA-binding protein. We compared the expression of an Alr3614-deletion strain with an Alr3614-complementation strain.
Project description:Cyanoglobules (CGs) are thylakoid-associated lipid droplets in cyanobacteria that share compositional and functional features with plastoglobules (PGs) of plant chloroplasts. However, their roles in stress physiology remain poorly defined, particularly in filamentous cyanobacteria. Here, we characterize the CG proteome in a non-nitrogen-fixing (ΔN) strain of Anabaena sp. PCC 7120 under nitrogen deprivation, providing an unbiased framework to examine CG responses without the confounding influence of heterocyst differentiation or diazotrophy. Proteomic analysis revealed a conserved CG proteome enriched in plastoglobule homologs, redox regulators (NDC1, GRXC2), and isoprenoid metabolism enzymes (NDX1), supporting roles in pigment turnover and stress adaptation.