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Nuclear functions of CFAP20 in transcription and replication [BrU-seq]

ABSTRACT: Nuclear functions of CFAP20 in transcription and replication [BrU-seq]

PROVIDER: PRJNA1107610 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28891260_1.fastq.gz	Fastqsanger.gz
	SRR28891260_2.fastq.gz	Fastqsanger.gz
	SRR28891261_1.fastq.gz	Fastqsanger.gz
	SRR28891261_2.fastq.gz	Fastqsanger.gz
	SRR28891262_1.fastq.gz	Fastqsanger.gz

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Nuclear functions of CFAP20 in transcription and replication

Project description:Nuclear functions of CFAP20 in transcription and replication

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Nuclear functions of CFAP20 in transcription and replication [BrU-seq]

Project description:Fine-tuning DNA replication and transcription co-occurrence is vital to avoid collisions between their machineries. This is especially relevant near promoters, where RNAPII initiates transcription and often arrests, forming R-loops. Arrested RNAPII poses a roadblock for DNA replication, which evolutionarily initiates near promoters. The mechanisms that salvage arrested RNAPII during elongation to avoid conflicts with incoming replisomes remain unaddressed. In this study, employing genome-wide and proteomic approaches, we identify and characterize CFAP20 as part of a protective pathway that rescues arrested RNAPII in promoter-proximal regions, diverting it from the path of co-directional replisomes. CFAP20-deficient cells accumulate R-loops specifically near promoters, displaying defects in replication origin firing and fork progression. Co-depletion of the Mediator coactivator complex or removal of RNAPII engaged with R-loops rescues these replication phenotypes. Therefore, we propose that CFAP20 salvages arrested RNAPII under conditions of high Mediator-driven transcription to promote RNAPII elongation, thereby preventing collisions with co-directional replisomes.

2025-05-20 | GSE266570 | GEO

Nuclear functions of CFAP20 in transcription and replication [ChIP-seq TY-CFAP20]

Project description:Nuclear functions of CFAP20 in transcription and replication [ChIP-seq TY-CFAP20]

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Nuclear functions of CFAP20 in transcription and replication [FLASH-seq]

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Nuclear functions of CFAP20 in transcription and replication [ChIP-seq]

Project description:Nuclear functions of CFAP20 in transcription and replication [ChIP-seq]

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Nuclear functions of CFAP20 in transcription and replication [DRIP-seq]

Project description:Nuclear functions of CFAP20 in transcription and replication [DRIP-seq]