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Nuclear functions of CFAP20 in transcription and replication [ChIP-seq TY-CFAP20]

ABSTRACT: Nuclear functions of CFAP20 in transcription and replication [ChIP-seq TY-CFAP20]

PROVIDER: PRJNA1267042 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR33690718_1.fastq.gz	Fastqsanger.gz
	SRR33690718_2.fastq.gz	Fastqsanger.gz
	SRR33690719_1.fastq.gz	Fastqsanger.gz
	SRR33690719_2.fastq.gz	Fastqsanger.gz
	SRR33690720_1.fastq.gz	Fastqsanger.gz

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Nuclear functions of CFAP20 in transcription and replication [ChIP-seq TY-CFAP20]

Project description:Fine-tuning DNA replication and transcription co-occurrence is vital to avoid collisions between their machineries. This is especially relevant near promoters, where RNAPII initiates transcription and often arrests, forming R-loops. Arrested RNAPII poses a roadblock for DNA replication, which evolutionarily initiates near promoters. The mechanisms that salvage arrested RNAPII during elongation to avoid conflicts with incoming replisomes remain unaddressed. In this study, employing genome-wide and proteomic approaches, we identify and characterize CFAP20 as part of a protective pathway that rescues arrested RNAPII in promoter-proximal regions, diverting it from the path of co-directional replisomes. CFAP20-deficient cells accumulate R-loops specifically near promoters, displaying defects in replication origin firing and fork progression. Co-depletion of the Mediator coactivator complex or removal of RNAPII engaged with R-loops rescues these replication phenotypes. Therefore, we propose that CFAP20 salvages arrested RNAPII under conditions of high Mediator-driven transcription to promote RNAPII elongation, thereby preventing collisions with co-directional replisomes.

2025-05-28 | GSE297966 | GEO

Nuclear functions of CFAP20 in transcription and replication

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Nuclear functions of CFAP20 in transcription and replication [ChIP-seq]

Project description:Nuclear functions of CFAP20 in transcription and replication [ChIP-seq]