Project description:To explore the possible role of Nsd2 in kidney cancer, the gene expression profile of kisney tissues from KMN (Ksp-Cre; Myc OE/+; Nsd2 OE/+) and littermate KM (Ksp-Cre; Myc OE/+) mice was examined by RNA sequencing(RNA-Seq).
Project description:Acetaminophen (APAP) overdose can cause acute kidney injury (AKI), but its molecular mechanisms remain poorly understood and no effective treatments are currently available. In this study, we performed an integrated analysis combining transcriptomic, proteomic, and phosphoproteomic profiling of kidney tissues from APAP-treated mice to investigate the underlying molecular mechanisms and identify potential therapeutic targets. Ten-week-old male C57BL/6 mice were fasted overnight for 16 hours prior to APAP administration. AKI was induced by intraperitoneal injection of a high dose of APAP (300 mg/kg body weight) for 6 hours (n = 4), while control mice received an equivalent volume of PBS via intraperitoneal injection (n = 4). Kidney tissues were collected from both APAP-treated and control groups for downstream omics analyses.
Project description:Acetaminophen (APAP) overdose can lead to acute kidney injury (AKI), yet its molecular mechanisms remain unclear and no effective treatments are currently available. In this study, we combined transcriptomic, proteomic, and phosphoproteomic profiling of kidneys from APAP-exposed mice to explore molecular mechanisms and potential therapeutic strategies. Ten-week-old male C57BL/6 mice were fasted overnight for 16 hours prior to APAP treatment. Acute kidney injury was induced by intraperitoneal injection of APAP overdose (300 mg/kg body weight) for 6 hours (n = 4). Control mice received an equivalent volume of PBS via intraperitoneal injection (n = 4). Kidney tissues were subsequently collected from APAP-induced kidney injury mice and PBS-injected controls.
Project description:This dataset comprises analysis data obtained from the kidney tissues of six mice with sepsis induced by intraperitoneal injection of lipopolysaccharide (LPS) (n=6), as well as six mice with kidney injury secondary to sepsis (n=6). The kidney tissues were harvested 24 hours post the establishment of the mouse models, and subsequent sequencing was conducted utilizing mass spectrometry technology.
Project description:Rhodamin 123 is a dye which can be used to detect the activity of ABC transporters. We observed that after staining of KM-H2 Hodgkin lymphoma cells with Rhodamin 123, part of the cells rapidly eliminated the dye, while another part of the cells retained the dye for a longer time. We compared the transcriptome of KM-H2 Hodgkin lymphoma cells with high Rhodamin 123 efflux capacity and KM-H2 cells with low Rhodamin 123 efflux capacity.
Project description:Kidney tissues were collected from mice 2 weeks after BDL and sham operations. Membrane fractions of the kidney were prepared. After sample preparation, the proteome analysis was performed.