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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [TT-Seq]

ABSTRACT: KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [TT-Seq]

PROVIDER: PRJNA1108274 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28964435_1.fastq.gz	Fastqsanger.gz
	SRR28964435_2.fastq.gz	Fastqsanger.gz
	SRR28964436_1.fastq.gz	Fastqsanger.gz
	SRR28964436_2.fastq.gz	Fastqsanger.gz
	SRR28964437_1.fastq.gz	Fastqsanger.gz

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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [TT-Seq]

Project description:Signal-induced transcriptional programs regulate critical biological processes through the precise spatiotemporal activation of Immediate Early Genes (IEGs); however, the mechanisms of transcription induction are still unfolding. By combining an acute depletion system with high resolution genomics approaches to interrogate synchronized, temporal transcription, we reveal that KAP1/TRIM28 is a first responder that fulfills the temporal and heightened transcriptional demand of IEGs. Unexpectedly, acute KAP1 loss triggers an increase in RNA Polymerase II elongation kinetics during early stimulation time points. This elongation defect derails the normal progression through the transcriptional cycle during late stimulation time points, ultimately leading to decreased recruitment of the transcription apparatus for re-initiation and dampening IEG transcription. Collectively, KAP1 plays a counterintuitive role by negatively regulating transcription elongation to support full activation across multiple transcription cycles of genes critical for cell physiology and organismal functions.

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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [PRO-Seq]

Project description:KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [PRO-Seq]

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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [RNA-Seq]

Project description:KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [RNA-Seq]

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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [ChIP-Seq]

Project description:KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [ChIP-Seq]

| PRJNA1108273 | ENA

KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [ChIP-Seq]

2024-05-07 | GSE266691 | GEO

KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [RNA-Seq]

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KAP1 negatively regulates elongation kinetics to activate signal-induced transcription [PRO-Seq]

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KAP1 regulates elongation kinetics to activate signal-induced transcription

Project description:KAP1 regulates elongation kinetics to activate signal-induced transcription

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KAP1 negatively regulates RNA polymerase II elongation kinetics to activate signal-induced transcription.

Project description:Signal-induced transcriptional programs regulate critical biological processes through the precise spatiotemporal activation of Immediate Early Genes (IEGs); however, the mechanisms of transcription induction remain poorly understood. By combining an acute depletion system with several genomics approaches to interrogate synchronized, temporal transcription, we reveal that KAP1/TRIM28 is a first responder that fulfills the temporal and heightened transcriptional demand of IEGs. Acute KAP1 loss triggers an increase in RNA polymerase II elongation kinetics during early stimulation time points. This elongation defect derails the normal progression through the transcriptional cycle during late stimulation time points, ultimately leading to decreased recruitment of the transcription apparatus for re-initiation thereby dampening IEGs transcriptional output. Collectively, KAP1 plays a counterintuitive role by negatively regulating transcription elongation to support full activation across multiple transcription cycles of genes critical for cell physiology and organismal functions.

| S-EPMC11245487 | biostudies-literature

Project description:tt

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